Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes

Daniel W. Pack; Frances H. Arnold

doi:10.1016/S0009-3084(97)02662-5

Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes

Daniel W. Pack, Frances H. Arnold

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Targeting and organization of proteins on lipid membranes led to applications in both biological and materials sciences. Coordination of membrane-bound metal ions by surface histidine residues provides a general method for targeting of proteins to membrane surfaces. Here we report the Langmuir monolayer properties of a new class of metal-chelating lipids. The lipids utilize the metal chelator iminodiacetate (IDA) as the hydrophilic headgroup, allowing display of divalent transition metal ions on the aqueous side of the membrane. Changes in surface pressure-molecular area isotherms were used to observe metal binding, and an association constant for Cu²⁺ binding to the IDA lipids of 10^7-8 M^-1 was estimated. The ability to control binding site density is important for many applications. The IDA lipid was found to be miscible with both distearoylphosphocholine (DSPC) and 1-stearoyl-2-oleoyl-phosphocholine (SOPC) at most compositions and surface pressures.

Original language	English
Pages (from-to)	135-152
Number of pages	18
Journal	Chemistry and Physics of Lipids
Volume	86
Issue number	2
DOIs	https://doi.org/10.1016/S0009-3084(97)02662-5
State	Published - Apr 25 1997

Bibliographical note

Funding Information:
The DPIDA lipid was kindly synthesized by Dr. Chao-Tsen Chen. This work was supported by the Office of Naval Research (N00014-92-J-1178). D.W.P. was a Landau fellow and is supported by a training fellowship from the National Institute of General Medical Sciences, NRSA Award 1 T32 GM 08346-01.

Funding

The DPIDA lipid was kindly synthesized by Dr. Chao-Tsen Chen. This work was supported by the Office of Naval Research (N00014-92-J-1178). D.W.P. was a Landau fellow and is supported by a training fellowship from the National Institute of General Medical Sciences, NRSA Award 1 T32 GM 08346-01.

Funders	Funder number
Office of Naval Research	N00014-92-J-1178
National Institute of General Medical Sciences	T32GM008346
Israel National Road Safety Authority	1 T32 GM 08346-01

Keywords

Fluorescence microscopy
Functionalized surfaces
Iminodiacetate-copper
Molecular recognition
Protein immobilization

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Organic Chemistry
Cell Biology

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10.1016/S0009-3084(97)02662-5

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title = "Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes",

abstract = "Targeting and organization of proteins on lipid membranes led to applications in both biological and materials sciences. Coordination of membrane-bound metal ions by surface histidine residues provides a general method for targeting of proteins to membrane surfaces. Here we report the Langmuir monolayer properties of a new class of metal-chelating lipids. The lipids utilize the metal chelator iminodiacetate (IDA) as the hydrophilic headgroup, allowing display of divalent transition metal ions on the aqueous side of the membrane. Changes in surface pressure-molecular area isotherms were used to observe metal binding, and an association constant for Cu2+ binding to the IDA lipids of 107-8 M-1 was estimated. The ability to control binding site density is important for many applications. The IDA lipid was found to be miscible with both distearoylphosphocholine (DSPC) and 1-stearoyl-2-oleoyl-phosphocholine (SOPC) at most compositions and surface pressures.",

keywords = "Fluorescence microscopy, Functionalized surfaces, Iminodiacetate-copper, Molecular recognition, Protein immobilization",

author = "Pack, {Daniel W.} and Arnold, {Frances H.}",

note = "Funding Information: The DPIDA lipid was kindly synthesized by Dr. Chao-Tsen Chen. This work was supported by the Office of Naval Research (N00014-92-J-1178). D.W.P. was a Landau fellow and is supported by a training fellowship from the National Institute of General Medical Sciences, NRSA Award 1 T32 GM 08346-01. ",

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AU - Arnold, Frances H.

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PY - 1997/4/25

Y1 - 1997/4/25

N2 - Targeting and organization of proteins on lipid membranes led to applications in both biological and materials sciences. Coordination of membrane-bound metal ions by surface histidine residues provides a general method for targeting of proteins to membrane surfaces. Here we report the Langmuir monolayer properties of a new class of metal-chelating lipids. The lipids utilize the metal chelator iminodiacetate (IDA) as the hydrophilic headgroup, allowing display of divalent transition metal ions on the aqueous side of the membrane. Changes in surface pressure-molecular area isotherms were used to observe metal binding, and an association constant for Cu2+ binding to the IDA lipids of 107-8 M-1 was estimated. The ability to control binding site density is important for many applications. The IDA lipid was found to be miscible with both distearoylphosphocholine (DSPC) and 1-stearoyl-2-oleoyl-phosphocholine (SOPC) at most compositions and surface pressures.

AB - Targeting and organization of proteins on lipid membranes led to applications in both biological and materials sciences. Coordination of membrane-bound metal ions by surface histidine residues provides a general method for targeting of proteins to membrane surfaces. Here we report the Langmuir monolayer properties of a new class of metal-chelating lipids. The lipids utilize the metal chelator iminodiacetate (IDA) as the hydrophilic headgroup, allowing display of divalent transition metal ions on the aqueous side of the membrane. Changes in surface pressure-molecular area isotherms were used to observe metal binding, and an association constant for Cu2+ binding to the IDA lipids of 107-8 M-1 was estimated. The ability to control binding site density is important for many applications. The IDA lipid was found to be miscible with both distearoylphosphocholine (DSPC) and 1-stearoyl-2-oleoyl-phosphocholine (SOPC) at most compositions and surface pressures.

KW - Fluorescence microscopy

KW - Functionalized surfaces

KW - Iminodiacetate-copper

KW - Molecular recognition

KW - Protein immobilization

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Langmuir monolayer characterization of metal chelating lipids for protein targeting to membranes

Abstract

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Keywords

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