Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

Nitai C. Hait; Katsuhide Fujita; Robert L. Lester; Robert C. Dickson

doi:10.1016/S0014-5793(02)03636-0

Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

Nitai C. Hait, Katsuhide Fujita, Robert L. Lester, Robert C. Dickson

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

Original language	English
Pages (from-to)	97-102
Number of pages	6
Journal	FEBS Letters
Volume	532
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(02)03636-0
State	Published - Dec 4 2002

Bibliographical note

Funding Information:
This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.

Keywords

Phytosphingosine
Sphingolipid
Sphingosine kinase
Sphingosine-1-phosphate

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/S0014-5793(02)03636-0

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title = "Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes",

abstract = "Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.",

keywords = "Phytosphingosine, Sphingolipid, Sphingosine kinase, Sphingosine-1-phosphate",

author = "Hait, {Nitai C.} and Katsuhide Fujita and Lester, {Robert L.} and Dickson, {Robert C.}",

note = "Funding Information: This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.",

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AU - Hait, Nitai C.

AU - Fujita, Katsuhide

AU - Lester, Robert L.

AU - Dickson, Robert C.

N1 - Funding Information: This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.

PY - 2002/12/4

Y1 - 2002/12/4

N2 - Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

AB - Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

KW - Phytosphingosine

KW - Sphingolipid

KW - Sphingosine kinase

KW - Sphingosine-1-phosphate

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Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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