Lcb4p sphingoid base kinase localizes to the Golgi and late endosomes

Nitai C. Hait, Katsuhide Fujita, Robert L. Lester, Robert C. Dickson

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Sphingoid long chain base phosphates (LCBPs) regulate cell proliferation, survival and motility in mammals. To learn more about LCBPs in Saccharomyces cerevisiae, we determined the cellular location of Lcb4p, the major enzyme catalyzing LCBP synthesis. By indirect immunofluorescence microscopy and subcellular fractionation, Lcb4p localizes to the trans-Golgi network and late endosomes and cycles between these compartments. Lcb4p faces the cytosol and is probably bound to membranes by protein-protein interactions. These results indicate that LCBs made in the endoplasmic reticulum must transit to the Golgi to be converted into LCBPs, which must then return to the endoplasmic reticulum to be degraded.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalFEBS Letters
Issue number1-2
StatePublished - Dec 4 2002

Bibliographical note

Funding Information:
This work was supported by Public Health Service Grant GM-58507 from the National Institutes of General Medical Sciences. We thank Drs. H. Pelham, S. Munro, P. Novick, and R. Schekman for antibodies and Dr. S. Nwaka for assistance in generating anti-Lcb4p antibodies and plasmids expressing Lcb4p.


  • Phytosphingosine
  • Sphingolipid
  • Sphingosine kinase
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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