Learning based automatic detection of myonuclei in isolated single skeletal muscle fibers using multi-focus image fusion

Hai Su, Fuyong Xing, Jonah D. Lee, Charlotte A. Peterson, Lin Yang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

8 Scopus citations

Abstract

Accurate and robust detection of myonuclei in single muscle fiber is required to calculate myonuclear domain size. However, this task is challenging because: 1) The myonuclei have a variety of sizes and shapes. 2) Imaging techniques exhibit myonuclei that are often overlapping. 3) Inhomogeneous intensity due to DAPI concentration in heterochromatin, abundant in mouse nuclei, results in a speckled appearance inside each myonucleus. In this paper, we propose a novel automatic approach to robustly detect the myonuclei in isolated single skeletal muscle fibers. The original z-stack images are first fused into one all-in-focus image. A sufficient number of ellipse fitting hypotheses are then generated using the myonuclei contour segments. A set of morphological features are calculated from the ellipses and utilized to train a support vector machine (SVM) classifier to choose the best candidates. A modified inner geodesic distance based clustering algorithm is used to produce the final results. The proposed method was extensively tested using 42 sets of z-stack images containing about 1500 myonuclei. The method demonstrates excellent results outperforming current state-of-the-arts.

Original languageEnglish
Title of host publicationISBI 2013 - 2013 IEEE 10th International Symposium on Biomedical Imaging
Subtitle of host publicationFrom Nano to Macro
Pages432-435
Number of pages4
DOIs
StatePublished - 2013
Event2013 IEEE 10th International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2013 - San Francisco, CA, United States
Duration: Apr 7 2013Apr 11 2013

Publication series

NameProceedings - International Symposium on Biomedical Imaging
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Conference

Conference2013 IEEE 10th International Symposium on Biomedical Imaging: From Nano to Macro, ISBI 2013
Country/TerritoryUnited States
CitySan Francisco, CA
Period4/7/134/11/13

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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