Ligand Competition Binding Assay for the Androgen Receptor

Clémentine Féau; Leggy A. Arnold; Aaron Kosinski; R. Kiplin Guy

doi:10.1007/978-1-61779-243-4_4

Ligand Competition Binding Assay for the Androgen Receptor

Clémentine Féau, Leggy A. Arnold, Aaron Kosinski, R. Kiplin Guy

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Scopus citations

Abstract

Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.

Original language	English
Title of host publication	Androgen Action
Subtitle of host publication	Methods and Protocols
Pages	59-68
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-61779-243-4_4
State	Published - 2011

Publication series

Name	Methods in Molecular Biology
Volume	776
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
This work was supported by the American Lebanese Syrian Associated Charities (ALSAC), St. Jude Children’s Research Hospital (SJCRH), the NIH (DK58080) and the Department of Defense Prostate Cancer Research Program (PC060344-W81XWH-07-1-0073).

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.

Keywords

Androgen receptor
endocrine disrupting chemicals
high-throughput screening
hormone competition assay
nuclear receptor
scintillation proximity assay

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-61779-243-4_4

Cite this

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abstract = "Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.",

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AU - Kosinski, Aaron

AU - Guy, R. Kiplin

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Ligand Competition Binding Assay for the Androgen Receptor

Abstract

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Bibliographical note

Keywords

ASJC Scopus subject areas

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