Ligand Competition Binding Assay for the Androgen Receptor

Clémentine Féau, Leggy A. Arnold, Aaron Kosinski, R. Kiplin Guy

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Scopus citations


Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.

Original languageEnglish
Title of host publicationAndrogen Action
Subtitle of host publicationMethods and Protocols
Number of pages10
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Funding Information:
This work was supported by the American Lebanese Syrian Associated Charities (ALSAC), St. Jude Children’s Research Hospital (SJCRH), the NIH (DK58080) and the Department of Defense Prostate Cancer Research Program (PC060344-W81XWH-07-1-0073).

Publisher Copyright:
© 2011, Springer Science+Business Media, LLC.


  • Androgen receptor
  • endocrine disrupting chemicals
  • high-throughput screening
  • hormone competition assay
  • nuclear receptor
  • scintillation proximity assay

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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