Evaluating endocrine activities of environmental chemicals or screening for new small molecule modulators of the androgen receptor (AR) transcription activity requires standardized and reliable assay procedures. Scintillation proximity assays (SPA) are sensitive and reliable techniques that are suitable for ligand competition binding assays. We have utilized a radiolabeled ligand competition binding assay for the androgen receptor (AR) that can be carried out in a 384-well format. This standardized, highly reproducible and low-cost assay has been automated for high-throughput screening (HTS) purposes.
|Title of host publication||Androgen Action|
|Subtitle of host publication||Methods and Protocols|
|Number of pages||10|
|State||Published - 2011|
|Name||Methods in Molecular Biology|
Bibliographical noteFunding Information:
This work was supported by the American Lebanese Syrian Associated Charities (ALSAC), St. Jude Children’s Research Hospital (SJCRH), the NIH (DK58080) and the Department of Defense Prostate Cancer Research Program (PC060344-W81XWH-07-1-0073).
© 2011, Springer Science+Business Media, LLC.
- Androgen receptor
- endocrine disrupting chemicals
- high-throughput screening
- hormone competition assay
- nuclear receptor
- scintillation proximity assay
ASJC Scopus subject areas
- Molecular Biology