Ligand-induced reorganization and assembly in synthetic lipid membranes

Daniel W. Pack, Kingman Ng, Kevin M. Maloney, Frances H. Arnold

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Membrane targetting of soluble ligands accompanied by assembly of membrane components into functional superstructures underlies biological signal transduction and a variety of other processes ranging from blood coagulation to biomineralization. Protein or lipid components provide the interactions required for targetting and specific orientation of bound molecules; the membrane's fluidity allows reorganization and sampling of intermolecular contacts required for assembly into superstructures. We are developing synthetic membrane-based recognition systems capable of reproducing important features of biological targetting and assembly. Systems such as these may open up new routes to controlling molecular architecture in materials and devices. Specially designed metal-chelating receptor/reporter lipids have been used to study lipid reorganization induced by binding of metal-complexing ligands. Proteins and peptides are targetted to the Cu2+ - and Ni2+ -complexing lipids via coordination interactions with surface histidines. Binding and assembly of multivalent ligands are accompanied by reorganization of the lipid receptors, as measured by fluorescence spectroscopy and fluorescence microscopy. Coordination interactions between protein and chelating lipid components can be used for direct assembly into superstructures such as patterned lipid monolayers and two-dimensional protein crystals.

Original languageEnglish
Pages (from-to)3-10
Number of pages8
JournalSupramolecular Science
Issue number1-2
StatePublished - 1997

Bibliographical note

Funding Information:
We would like to thank our collaboratorso n the 2D crystallizationo f streptavidinV, iola Vogel and Patrick Stayton, Center for Bioengineering, University of Washington, Seattle, WA, USA. This work is supported by the Office of Naval Research( N00014-924-1178)a nd the Army ResearchO ffice.

ASJC Scopus subject areas

  • Engineering (all)


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