Linker histone H1 and H3K56 acetylation are antagonistic regulators of nucleosome dynamics

Morgan Bernier, Yi Luo, Kingsley C. Nwokelo, Michelle Goodwin, Sarah J. Dreher, Pei Zhang, Mark R. Parthun, Yvonne Fondufe-Mittendorf, Jennifer J. Ottesen, Michael G. Poirier

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

H1 linker histones are highly abundant proteins that compact nucleosomes and chromatin to regulate DNA accessibility and transcription. However, the mechanisms that target H1 regulation to specific regions of eukaryotic genomes are unknown. Here we report fluorescence measurements of human H1 regulation of nucleosome dynamics and transcription factor (TF) binding within nucleosomes. H1 does not block TF binding, instead it suppresses nucleosome unwrapping to reduce DNA accessibility within H1-bound nucleosomes. We then investigated H1 regulation by H3K56 and H3K122 acetylation, two transcriptional activating histone post translational modifications (PTMs). Only H3K56 acetylation, which increases nucleosome unwrapping, abolishes H1.0 reduction of TF binding. These findings show that nucleosomes remain dynamic, while H1 is bound and H1 dissociation is not required for TF binding within the nucleosome. Furthermore, our H3K56 acetylation measurements suggest that a single-histone PTM can define regions of the genome that are not regulated by H1.

Original languageEnglish
Article number10152
JournalNature Communications
Volume6
DOIs
StatePublished - Dec 9 2015

Bibliographical note

Funding Information:
We thank Justin North for help with nucleosome sample preparation and the initial H1.0 titrations. We thank John C. Shimko for assistance with modified histone preparation and analysis. This research is supported by the NIH grants R01 GM083055 (to M.G.P. and J.J.O.), R01 ES024478 (to Y.F.M.) and R01 GM62970 (to M.R.P.), and the NSF grant 1517986 (to Y.F.M.).

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry, Genetics and Molecular Biology
  • General Physics and Astronomy

Fingerprint

Dive into the research topics of 'Linker histone H1 and H3K56 acetylation are antagonistic regulators of nucleosome dynamics'. Together they form a unique fingerprint.

Cite this