Abstract
Exposure to albumin-bound linoleic acid (60 to 150 μM) for 24 h significantly increased the rate of albumin transfer across cultured endothelial monolayers. The increase was dependent on the linoleic acid (18:2) concentration to which the cultures were exposed. Linoleic acid hydroperoxide (18:2-OOH) further accelerated the rate of albumin transfer over that of 18:2. A near maximum albumin transfer was observed after a 2-h incubation with 90 μM 18:2-OOH. Cells exposed to 18:2-OOH caused a marked release of lactate dehydrogenase into the media. On the other hand, 18:2 at concentrations as high as 150 μM, did not significantly affect lactate dehydrogenase release. These results suggest that exposure to 18:2, and in particular to 18:2-OOH, reduces the ability of the endothelium to act as a selective permeability barrier to plasma components.
| Original language | English |
|---|---|
| Pages (from-to) | 353-357 |
| Number of pages | 5 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 248 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1986 |
Bibliographical note
Funding Information:i Supported in part by grant HL 34423 from the National Heart, Lung, and Blood Institute, National Institutes of Health, a grant from the American Heart Association, Kentucky Affiliate; a University of Kentucky Biological Research Support grant; and the Kentucky Agricultural Experiment Station. ‘To whom correspondence should be addressed.
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology