TY - JOUR
T1 - Lipase-catalyzed process for biodiesel production
T2 - Protein engineering and lipase production
AU - Hwang, Hyun Tae
AU - Qi, Feng
AU - Yuan, Chongli
AU - Zhao, Xuebing
AU - Ramkrishna, Doraiswami
AU - Liu, Dehua
AU - Varma, Arvind
PY - 2014/4
Y1 - 2014/4
N2 - Biodiesel is an environment-friendly and renewable fuel produced by transesterification of various feedstocks. Although the lipase-catalyzed biodiesel production has many advantages over the conventional alkali catalyzed process, its industrial applications have been limited by high-cost and low-stability of lipase enzymes. This review provides a general overview of the recent advances in lipase engineering, including both protein modification and production. Recent advances in biotechnology such as in protein engineering, recombinant methods and metabolic engineering have been employed but are yet to impact lipase engineering for cost-effective production of biodiesel. A summary of the current challenges and perspectives for potential solutions are also provided. Biotechnol. Bioeng. 2014;111: 639-653.
AB - Biodiesel is an environment-friendly and renewable fuel produced by transesterification of various feedstocks. Although the lipase-catalyzed biodiesel production has many advantages over the conventional alkali catalyzed process, its industrial applications have been limited by high-cost and low-stability of lipase enzymes. This review provides a general overview of the recent advances in lipase engineering, including both protein modification and production. Recent advances in biotechnology such as in protein engineering, recombinant methods and metabolic engineering have been employed but are yet to impact lipase engineering for cost-effective production of biodiesel. A summary of the current challenges and perspectives for potential solutions are also provided. Biotechnol. Bioeng. 2014;111: 639-653.
KW - Biodiesel
KW - Enzyme
KW - Lipase
KW - Lipase production
KW - Protein engineering
KW - Transesterification
UR - http://www.scopus.com/inward/record.url?scp=84894281258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84894281258&partnerID=8YFLogxK
U2 - 10.1002/bit.25162
DO - 10.1002/bit.25162
M3 - Review article
C2 - 24284881
AN - SCOPUS:84894281258
SN - 0006-3592
VL - 111
SP - 639
EP - 653
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 4
ER -
