Lipid components that reduce protein solubility of soy protein isolates

W. L. Boatright; N. S. Hettiarachchy

doi:10.1007/BF02577836

Lipid components that reduce protein solubility of soy protein isolates

W. L. Boatright, N. S. Hettiarachchy

Animal and Food Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A lipid fraction from a commercial soy protein isolate (SPI), previously found to be detrimental to SPI solubility, was analyzed by size-exclusion liquid chromatography, by high-performance liquid chromatography (HPLC), and for chemical composition. The molecular weight of most of this material was greater than 1,100 daltons. This lipid fraction was water-soluble yet required a strong nonpolar solvent mixture to elute it from a C₁₈ HPLC column. The lipid material was alkaline (pH 8.7) and composed of 3.0% nitrogen, 1.6% phosphorus, 17.5% nonvolatile crude fatty acids primarily hydroxylated), 10.4% long-chain bases, 9.9% hexuronic acid, 3.2% hexosamine, and 6.6% total sugar. The molecular weight, chemical composition, and physical characteristics (solubility characteristics, surfactant characteristics, and appearance) of this material were all similar to those reported for phytoglycolipid.

Original language	English
Pages (from-to)	1445-1451
Number of pages	7
Journal	Journal of the American Oil Chemists' Society
Volume	72
Issue number	12
DOIs	https://doi.org/10.1007/BF02577836
State	Published - Dec 1995

Keywords

High-performance liquid chromatography
lipids
phytoglycolipid
size-exclusion chromatography
solubility
soy protein isolate

ASJC Scopus subject areas

General Chemical Engineering
Organic Chemistry

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10.1007/BF02577836

Cite this

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title = "Lipid components that reduce protein solubility of soy protein isolates",

abstract = "A lipid fraction from a commercial soy protein isolate (SPI), previously found to be detrimental to SPI solubility, was analyzed by size-exclusion liquid chromatography, by high-performance liquid chromatography (HPLC), and for chemical composition. The molecular weight of most of this material was greater than 1,100 daltons. This lipid fraction was water-soluble yet required a strong nonpolar solvent mixture to elute it from a C18 HPLC column. The lipid material was alkaline (pH 8.7) and composed of 3.0% nitrogen, 1.6% phosphorus, 17.5% nonvolatile crude fatty acids primarily hydroxylated), 10.4% long-chain bases, 9.9% hexuronic acid, 3.2% hexosamine, and 6.6% total sugar. The molecular weight, chemical composition, and physical characteristics (solubility characteristics, surfactant characteristics, and appearance) of this material were all similar to those reported for phytoglycolipid.",

keywords = "High-performance liquid chromatography, lipids, phytoglycolipid, size-exclusion chromatography, solubility, soy protein isolate",

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year = "1995",

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AU - Boatright, W. L.

AU - Hettiarachchy, N. S.

PY - 1995/12

Y1 - 1995/12

N2 - A lipid fraction from a commercial soy protein isolate (SPI), previously found to be detrimental to SPI solubility, was analyzed by size-exclusion liquid chromatography, by high-performance liquid chromatography (HPLC), and for chemical composition. The molecular weight of most of this material was greater than 1,100 daltons. This lipid fraction was water-soluble yet required a strong nonpolar solvent mixture to elute it from a C18 HPLC column. The lipid material was alkaline (pH 8.7) and composed of 3.0% nitrogen, 1.6% phosphorus, 17.5% nonvolatile crude fatty acids primarily hydroxylated), 10.4% long-chain bases, 9.9% hexuronic acid, 3.2% hexosamine, and 6.6% total sugar. The molecular weight, chemical composition, and physical characteristics (solubility characteristics, surfactant characteristics, and appearance) of this material were all similar to those reported for phytoglycolipid.

AB - A lipid fraction from a commercial soy protein isolate (SPI), previously found to be detrimental to SPI solubility, was analyzed by size-exclusion liquid chromatography, by high-performance liquid chromatography (HPLC), and for chemical composition. The molecular weight of most of this material was greater than 1,100 daltons. This lipid fraction was water-soluble yet required a strong nonpolar solvent mixture to elute it from a C18 HPLC column. The lipid material was alkaline (pH 8.7) and composed of 3.0% nitrogen, 1.6% phosphorus, 17.5% nonvolatile crude fatty acids primarily hydroxylated), 10.4% long-chain bases, 9.9% hexuronic acid, 3.2% hexosamine, and 6.6% total sugar. The molecular weight, chemical composition, and physical characteristics (solubility characteristics, surfactant characteristics, and appearance) of this material were all similar to those reported for phytoglycolipid.

KW - High-performance liquid chromatography

KW - lipids

KW - phytoglycolipid

KW - size-exclusion chromatography

KW - solubility

KW - soy protein isolate

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Lipid components that reduce protein solubility of soy protein isolates

Abstract

Keywords

ASJC Scopus subject areas

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