Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Joseph W. Wyse; D. Allan Butterfield

doi:10.1080/00032718808055501

Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Joseph W. Wyse, D. Allan Butterfield

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

Original language	English
Pages (from-to)	1131-1140
Number of pages	10
Journal	Analytical Letters
Volume	21
Issue number	7
DOIs	https://doi.org/10.1080/00032718808055501
State	Published - Jan 1988

Bibliographical note

Funding Information:
We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

Keywords

CAT-16
Spin-labeling
cationic spin label
erythrocyte membranes
lipid bilayer
lipid-protein interactions
membrane glycoconjugate

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032718808055501

Cite this

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title = "Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16",

abstract = "A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.",

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note = "Funding Information: We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.",

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language = "English",

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T2 - Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

AU - Wyse, Joseph W.

AU - Allan Butterfield, D.

N1 - Funding Information: We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

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Y1 - 1988/1

N2 - A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

AB - A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

KW - CAT-16

KW - Spin-labeling

KW - cationic spin label

KW - erythrocyte membranes

KW - lipid bilayer

KW - lipid-protein interactions

KW - membrane glycoconjugate

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JO - Analytical Letters

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Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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