Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Joseph W. Wyse; D. Allan Butterfield

doi:10.1080/00032718808055501

Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Joseph W. Wyse, D. Allan Butterfield

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

Original language	English
Pages (from-to)	1131-1140
Number of pages	10
Journal	Analytical Letters
Volume	21
Issue number	7
DOIs	https://doi.org/10.1080/00032718808055501
State	Published - Jan 1988

Bibliographical note

Funding Information:
We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

Funding

We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

Funders	Funder number
Tobacco and Health Research Institute
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China	RII-86-10671
U.S. Department of Energy Chinese Academy of Sciences Guangzhou Municipal Science and Technology Project Oak Ridge National Laboratory Extreme Science and Engineering Discovery Environment National Science Foundation National Energy Research Scientific Computing Center National Natural Science Foundation of China

Keywords

CAT-16
Spin-labeling
cationic spin label
erythrocyte membranes
lipid bilayer
lipid-protein interactions
membrane glycoconjugate

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032718808055501

Cite this

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title = "Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16",

abstract = "A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.",

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author = "Wyse, \{Joseph W.\} and \{Allan Butterfield\}, D.",

note = "Funding Information: We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.",

year = "1988",

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doi = "10.1080/00032718808055501",

language = "English",

volume = "21",

pages = "1131--1140",

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T1 - Lipid Specific Spin-Labeling of Erythrocyte Membranes

T2 - Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

AU - Wyse, Joseph W.

AU - Allan Butterfield, D.

N1 - Funding Information: We gratefully thank Us.M ary Anne Yacko for technical assistance and Pmfessor D. P. Diadrich for performing maphological experiments. This work was supported in part by grants h n NSF (RII-86-10671), DOD.and the Tobacco and Health Research Institute.

PY - 1988/1

Y1 - 1988/1

N2 - A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

AB - A new method for spin labeling the lipid bilayer component in erythrocyte membranes at low hematocrit with extremely low concentration of 4-(N,N-dimethyl-1-n-hexadecyl)ammonium-2,2,6,6-teiramethylpiperidine-1-oxy) iodide, (CAT-16) is described. Characterization of the motional determinants of CAT-16 in erythrocyte membranes at 20°C is presented from which it is deduced that protein-lipid interactions in the lipid bilayer are primary.

KW - CAT-16

KW - Spin-labeling

KW - cationic spin label

KW - erythrocyte membranes

KW - lipid bilayer

KW - lipid-protein interactions

KW - membrane glycoconjugate

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DO - 10.1080/00032718808055501

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SN - 0003-2719

VL - 21

SP - 1131

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JO - Analytical Letters

JF - Analytical Letters

IS - 7

ER -

Lipid Specific Spin-Labeling of Erythrocyte Membranes: Development and Characterization of a New Labeling Procedure for a Cationic Spin Label, Cat-16

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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