Listeria monocytogenes: Cultivation and laboratory maintenance

Grant S. Jones, Sarah E.F. D'Orazio

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

This unit describes general procedures for the lab cultivation and storage of the Grampositive facultative intracellular bacterium Listeria monocytogenes. The basic protocols are relevant for a wide scope of applications including microbial genetics and both in vitro and in vivo infection studies. Commonly used L. monocytogenes strains, serotypes, and growth parameters are also discussed.

Original languageEnglish
Article number9B.2
JournalCurrent Protocols in Microbiology
Issue numberSUPPL.31
DOIs
StatePublished - 2013

Keywords

  • Gram-positive
  • Intracellular pathogen
  • Listeria monocytogenes
  • Listeriosis

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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