Live-cell imaging of F-actin dynamics during fertilization in Arabidopsis thaliana

Daichi Susaki, Daisuke Maruyama, Ramesh Yelagandula, Frederic Berger, Tomokazu Kawashima

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

5 Scopus citations


Fertilization comprises a complex series of cellular processes leading to the fusion of a male and female gamete. Many studies have been carried out to investigate each step of fertilization in plants; however, our comprehensive understanding of all the sequential events during fertilization is still limited. This is largely due to difficulty in investigating events in the female gametophyte, which is deeply embedded in the maternal tissue. Recent advances in confocal microcopy assisted by fluorescent marker lines have contributed to visualizing subcellular dynamics in real time during fertilization in vivo. In this chapter, we describe a method focusing on the investigation of F-actin dynamics in the central cell during male gamete nuclear migration. This method also allows the study of a wide range of early sexual reproduction events, from pollen tube guidance to the early stage of seed development.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Number of pages8
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.


  • Arabidopsis
  • Confocal microscopy
  • F-actin
  • Fertilization
  • Gamete nuclear migration
  • Live-cell imaging

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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