Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures

Christopher I. Richards; Khai Luong; Rahul Srinivasan; Stephen W. Turner; Dennis A. Dougherty; Jonas Korlach; Henry A. Lester

doi:10.1021/nl301480h

Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures

Christopher I. Richards, Khai Luong, Rahul Srinivasan, Stephen W. Turner, Dennis A. Dougherty, Jonas Korlach, Henry A. Lester

Chemistry

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.

Original language	English
Pages (from-to)	3690-3694
Number of pages	5
Journal	Nano Letters
Volume	12
Issue number	7
DOIs	https://doi.org/10.1021/nl301480h
State	Published - Jul 11 2012

Keywords

Membrane receptors
nanostructures
nicotinic receptors
single-molecule imaging
zero-mode waveguide

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl301480h

Cite this

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title = "Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures",

abstract = "We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.",

keywords = "Membrane receptors, nanostructures, nicotinic receptors, single-molecule imaging, zero-mode waveguide",

author = "Richards, {Christopher I.} and Khai Luong and Rahul Srinivasan and Turner, {Stephen W.} and Dougherty, {Dennis A.} and Jonas Korlach and Lester, {Henry A.}",

year = "2012",

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doi = "10.1021/nl301480h",

language = "English",

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T1 - Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures

AU - Richards, Christopher I.

AU - Luong, Khai

AU - Srinivasan, Rahul

AU - Turner, Stephen W.

AU - Dougherty, Dennis A.

AU - Korlach, Jonas

AU - Lester, Henry A.

PY - 2012/7/11

Y1 - 2012/7/11

N2 - We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.

AB - We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.

KW - Membrane receptors

KW - nanostructures

KW - nicotinic receptors

KW - single-molecule imaging

KW - zero-mode waveguide

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DO - 10.1021/nl301480h

M3 - Article

C2 - 22668081

AN - SCOPUS:84863837224

SN - 1530-6984

VL - 12

SP - 3690

EP - 3694

JO - Nano Letters

JF - Nano Letters

IS - 7

ER -

Live-cell imaging of single receptor composition using zero-mode waveguide nanostructures

Abstract

Keywords

ASJC Scopus subject areas

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