Abstract
Seed development in flowering plants is highly complex and governed by three genetically distinct tissues: the fertilization products, the diploid embryo and triploid endosperm, as well as the seed coat that has maternal origin. There are diverse cellular dynamics such as nuclear movement in gamete cells for fertilization, cell polarity establishment for embryo development, and multinuclear endosperm formation. These tissues also coordinate and synchronize the developmental timing for proper seed formation through cell-to-cell communications. Live-cell imaging using advanced microscopy techniques enables us to decipher the dynamics of these events. Especially, the establishment of a less-invasive semi-in vivo live-cell imaging approach has allowed us to perform time-lapse analyses for long period observation of Arabidopsis thaliana intact seed development dynamics. Here we highlight the recent trends of live-cell imaging for seed development and discuss where we are heading.
| Original language | English |
|---|---|
| Article number | 111485 |
| Journal | Plant Science |
| Volume | 325 |
| DOIs | |
| State | Published - Dec 2022 |
Bibliographical note
Funding Information:We are grateful to Dr. Anthony Clark for his critical comments on this manuscript. This work was supported by NSF Grant IOS-1928836 (to T.K.); National Institute of Food and Agriculture, US Department of Agriculture Hatch Program Grant 1014280 (to T.K.).
Publisher Copyright:
© 2022 Elsevier B.V.
Keywords
- Confocal microscopy
- Embryo
- Endosperm
- Fertilization
- Seed
- Semi-in vivo live-cell imaging
ASJC Scopus subject areas
- Genetics
- Agronomy and Crop Science
- Plant Science