Loading PEG-catalase into filamentous and spherical polymer nanocarriers

Eric A. Simone, Thomas D. Dziubla, Evguenia Arguiri, Vanessa Vardon, Vladimir V. Shuvaev, Melpo Christofidou-Solomidou, Vladimir R. Muzykantov

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Purpose. Based on a unique phase alignment that occurs during formulation, we postulated that PEG-ylation of the cargo enzyme would enhance its encapsulation within diblock copolymer nanocarriers and thus resistance to proteases. Methods. A freeze-thaw modified double emulsion technique was utilized to encapsulate either the catalytically active enzyme catalase (MW ∼250 kDa) or PEG-catalase in PEG-PLA polymer nanocarriers (PNC). Spectrophotometer measurement of substrate depletion was utilized to monitor enzyme activity. Isotope labeling of the enzyme was used in conjunction with activity measurements to determine PNC loading efficiency and PNC-enzyme resistance to proteases. This labeling also enabled blood clearance measurements of PNC-loaded and non-loaded enzymes in mice. Results. Non-loaded PEG-catalase exhibited longer circulation times than catalase, but was equally susceptible to proteolysis. Modulation of the ratio of relatively hydrophilic to hydrophobic domains in the diblock PEG-PLA copolymer provided either filamentous or spherical PNC loaded with PEG-catalase. For both PNC geometries, encapsulation and resistance to proteases of the resultant PNC-loaded enzyme were more effective for PEG-catalase than catalase. Isotope tracing showed similar blood levels of PNC-loaded and free PEG-catalase in mice. Conclusions. PEGylation enhances active catalase loading within PNC and resistance to protease degradation, relative to unloaded PEG-catalase.

Original languageEnglish
Pages (from-to)250-260
Number of pages11
JournalPharmaceutical Research
Volume26
Issue number1
DOIs
StatePublished - Jan 2009

Bibliographical note

Funding Information:
This work was supported by grants from the National Institutes of Health (NIH #’s HL007954, HL073940-01-A1, HL087036 and PO1-HL079063). We thank Tony Lowman and the Centralized Materials Characterization Facility and Industry Consortium of Drexel University for assistance with NMR and SEM studies and Dennis Discher of the University of Pennsylvania for GPC studies.

Keywords

  • Antioxidant enzymes
  • Encapsulation
  • Filamentous carrier
  • PEG-catalase
  • Polymer nanocarrier

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry
  • Pharmacology (medical)

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