Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry

J. E. Springer, E. Robbins, S. Meyer, F. Baldino, M. E. Lewis

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

1. In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a35S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalCellular and Molecular Neurobiology
Volume10
Issue number1
DOIs
StatePublished - Mar 1990

Keywords

  • NGF receptor (NGFR)
  • cholinergic neurons
  • in situ hybridization
  • nerve growth factor (NGF)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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