Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry

J. E. Springer; E. Robbins; S. Meyer; F. Baldino; M. E. Lewis

doi:10.1007/BF00733633

Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry

J. E. Springer, E. Robbins, S. Meyer, F. Baldino, M. E. Lewis

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

1. In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections³⁵S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a³⁵S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.

Original language	English
Pages (from-to)	33-39
Number of pages	7
Journal	Cellular and Molecular Neurobiology
Volume	10
Issue number	1
DOIs	https://doi.org/10.1007/BF00733633
State	Published - Mar 1990

Keywords

NGF receptor (NGFR)
cholinergic neurons
in situ hybridization
nerve growth factor (NGF)

ASJC Scopus subject areas

Cellular and Molecular Neuroscience
Cell Biology

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10.1007/BF00733633

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title = "Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry",

abstract = "1. In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a35S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.",

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author = "Springer, {J. E.} and E. Robbins and S. Meyer and F. Baldino and Lewis, {M. E.}",

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T1 - Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry

AU - Springer, J. E.

AU - Robbins, E.

AU - Meyer, S.

AU - Baldino, F.

AU - Lewis, M. E.

PY - 1990/3

Y1 - 1990/3

N2 - 1. In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a35S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.

AB - 1. In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a35S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.

KW - NGF receptor (NGFR)

KW - cholinergic neurons

KW - in situ hybridization

KW - nerve growth factor (NGF)

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Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry

Abstract

Keywords

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