TY - JOUR
T1 - Localization of nerve growth factor receptor mRNA in the rat basal forebrain with in situ hybridization histochemistry
AU - Springer, J. E.
AU - Robbins, E.
AU - Meyer, S.
AU - Baldino, F.
AU - Lewis, M. E.
PY - 1990/3
Y1 - 1990/3
N2 - 1. In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a35S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.
AB - 1. In situ hybridization histochemistry was used to localize nerve growth factor receptor (NGFR) mRNA in the adult rat basal forebrain. 2. In emulsion-dipped sections35S-labeled RNA antisense probes produced a high density of silver grains over cells located in the medial septum, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. 3. This distribution of NGFR mRNA overlaps with the distribution of NGFR protein localized using immunocytochemical techniques. 4. No hybridization signal was detected when sections were hybridized with a35S-labeled RNA sense (control) probe. 5. We suggest that NGFRs are synthesized in these basal forebrain nuclei and transported to terminal areas where NGF is thought to be bound and internalized, an initial step in the many actions of this neurotrophic factor.
KW - NGF receptor (NGFR)
KW - cholinergic neurons
KW - in situ hybridization
KW - nerve growth factor (NGF)
UR - https://www.scopus.com/pages/publications/0025125196
UR - https://www.scopus.com/pages/publications/0025125196#tab=citedBy
U2 - 10.1007/BF00733633
DO - 10.1007/BF00733633
M3 - Article
C2 - 2159382
AN - SCOPUS:0025125196
SN - 0272-4340
VL - 10
SP - 33
EP - 39
JO - Cellular and Molecular Neurobiology
JF - Cellular and Molecular Neurobiology
IS - 1
ER -