Localized aggressive periodontitis immune response to healthy and diseased subgingival plaque

Luciana M. Shaddox, William P. Spencer, Irina M. Velsko, Hiba Al-Kassab, Hong Huang, Nadia Calderon, Ikramuddin Aukhil, Shannon M. Wallet

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Aim: The objective of this case–control study was to compare the inflammatory response of peripheral blood from localized aggressive periodontitis (LAP) patients when stimulated with healthy or diseased plaque samples. Materials and Methods: Whole blood and subgingival plaque samples were collected from 13 LAP subjects, 14 siblings of LAP subjects and six periodontally healthy individuals. Whole blood was stimulated for 24 h with plaque samples generated from healthy or diseased sites. The levels of 14 cyto/chemokines were detected using multiplex technology. Results: Localized aggressive periodontitis-derived cultures displayed higher levels of G-CSF, INFγ, IL10, IL12p40, IL1β, IL-6, IL-8, MCP-1, MIP-1α, and TNFα, than control cultures regardless of stimulus used. Whole blood from healthy siblings displayed higher levels of IL-6 compared to control subjects, but lower levels than those observed in cultures from LAP participants. Conclusions: This study suggests that although bacteria is an important factor in eliciting the hyper-inflammatory response observed in LAP patients, the predisposition of host's response to bacterial presence may play a more significant role than the components of the stimulatory plaque.

Original languageEnglish
Pages (from-to)746-753
Number of pages8
JournalJournal of Clinical Periodontology
Volume43
Issue number9
DOIs
StatePublished - Sep 1 2016

Bibliographical note

Publisher Copyright:
© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Keywords

  • aggressive periodontitis
  • chemokines
  • cytokines
  • endotoxin
  • microbiology

ASJC Scopus subject areas

  • Periodontics

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