Long-term increase of Sp-1 transcription factors in the hippocampus after kainic acid treatment

Zhehui Feng, Raymond C.C. Chang, Guoying Bing, Pearlie Hudson, Ningyi Tiao, Lei Jin, Jau Shyong Hong

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Systemic administration of kainic acid (KA), a glutamate receptor agonist, causes robust seizures and has been used as an excellent rodent model for human temporal lobe epilepsy. Recently, we have demonstrated that a single injection of KA increases the steady-state levels of proenkephalin (PENK) mRNA in the rat hippocampus for at least one year. However, the molecular mechanisms underlying this long-term increase in PENK mRNA levels have not been clearly defined. To determine the possible involvement of the Sp-1 transcription factors in this regulation, electrophoresis mobility-shift assays were used to study the expression of Sp-1 factors in the hippocampus after KA treatment. The results showed that there are long-lasting increases in Sp-1 DNA-binding activity. The Sp-1 DNA-binding complexes were only competed by the non-radioactive Sp-1 element and not by ENKCRE2, AP-1 or CRE elements, indicating the specificity of Sp-1 DNA-binding activity. Since the expression of Sp-1 parallels the time course of long-lasting increase in the expression of PENK mRNA and mossy fiber sprouting after KA treatment, we hypothesize that the increase in Sp-1 activity may be associated with the long-term changes in the plasticity of hippocampal function after KA-induced seizures. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)144-148
Number of pages5
JournalMolecular Brain Research
Volume69
Issue number1
DOIs
StatePublished - May 21 1999

Keywords

  • Hippocampus
  • Kainic acid
  • Mossy fiber sprouting
  • Temporal lobe epilepsy
  • Transcription factor

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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