Long term storage of miRNA at room and elevated temperatures in a silica sol-gel matrix

Rajat Chauhan, Theodore S. Kalbfleisch, Chinmay S. Potnis, Meenakshi Bansal, Mark W. Linder, Robert S. Keynton, Gautam Gupta

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Storage of biospecimens in their near native environment at room temperature can have a transformative global impact, however, this remains an arduous challenge to date due to the rapid degradation of biospecimens over time. Currently, most isolated biospecimens are refrigerated for short-term storage and frozen (-20 °C, -80 °C, liquid nitrogen) for long-term storage. Recent advances in room temperature storage of purified biomolecules utilize anhydrobiosis. However, a near aqueous storage solution that can preserve the biospecimen nearly "as is"has not yet been achieved by any current technology. Here, we demonstrate an aqueous silica sol-gel matrix for optimized storage of biospecimens. Our technique is facile, reproducible, and has previously demonstrated stabilization of DNA and proteins, within a few minutes using a standard benchtop microwave. Herein, we demonstrate complete integrity of miRNA 21, a highly sensitive molecule at 4, 25, and 40 °C over a period of ∼3 months. In contrast, the control samples completely degrade in less than 1 week. We attribute excellent stability to entrapment of miRNA within silica-gel matrices.

Original languageEnglish
Pages (from-to)31505-31510
Number of pages6
JournalRSC Advances
Volume11
Issue number50
DOIs
StatePublished - Sep 8 2021

Bibliographical note

Publisher Copyright:
© 2021 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • General Chemistry
  • General Chemical Engineering

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