A panel of five monoclonal antibodies detecting human lymphocyte function-associated antigen 1 (LFA-1) was generated and shown by competitive binding studies to react with at least four distinct epitopes on this molecule. The antibodies were then tested for their ability to inhibit the lytic activity of a variety of different human natural killer (NK) populations on a panel of four NK-susceptible target cells (K562, MOLT-4, HSB-2, and Jurkat). When heterogeneous NK populations derived from fresh peripheral blood and mixed-lymphocyte culture (MLC)-generated lines were used, these anti-LFA-1 monoclonal antibodies (MAbs) inhibited lysis of all four NK targets; this finding supports the notion that LFA-1 molecules play an important role in NK-mediated lysis. When tested on a cloned line of NK cells (NK 3.3), lysis of K562 was inhibited by these MAbs, but lysis of the other three targets was not affected. This represents an instance where a MAb specific for LFA-1 inhibits the lytic activity of NK cells against some but not all targets; thus the LFA-1 molecule cannot be considered under all circumstances to be an absolute requirement in NK-mediated lysis.
|Number of pages||12|
|State||Published - Oct 15 1987|
Bibliographical noteFunding Information:
’ This work was supported by NIH Grants CA-37827, CA 15822, and CA-09 140 and funding from Cytogen Corp. * To whom correspondence should be addressed. 3 Current address: Medical Biology Institute, 11077 North Torrey Pines Rd., La Jolla, CA 92037. 4 Abbreviations used: ADCC, antibodydependent cell-mediated cytotoxicity; CTL, cytotoxic T lymphocytes; FBS, fetal bovine serum; HS, human serum; IL-2, interleukin-2; LFA-1, lymphocyte function-associated antigen 1; MAb, monoclonal antibody; 2-ME, 2-mercaptoethanol; MLC, mixed-lymphocyte culture; NK, natural killer cell; PBL, peripheral blood mononuclear leukocytes; PBS, phosphate-buffered saline; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide electrophoresis; StA, Staphylococcus aureus.
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