Macrophage effector responses of horses are influenced by expression of CD154

Brett A. Sponseller; Sandra K. Clark; Jessica Gilbertie; David M. Wong; Kate Hepworth; Sarah Wiechert; Prashanth Chandramani-Shivalingappa; Beatrice T. Sponseller; Cody J. Alcott; Bryan Bellaire; Andrew C. Petersen; Douglas E. Jones

doi:10.1016/j.vetimm.2016.08.001

Macrophage effector responses of horses are influenced by expression of CD154

Brett A. Sponseller, Sandra K. Clark, Jessica Gilbertie, David M. Wong, Kate Hepworth, Sarah Wiechert, Prashanth Chandramani-Shivalingappa, Beatrice T. Sponseller, Cody J. Alcott, Bryan Bellaire, Andrew C. Petersen, Douglas E. Jones

Veterinary Science

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Reactive intermediates contribute to innate immunity by providing phagocytes with a mechanism of defense against bacteria, viruses and parasites. To better characterize the role of CD154 in the production of reactive intermediates, we cloned and expressed recombinant equine CD154 (reqCD154) in Chinese Hamster Ovary (CHO). In co-culture experiments, CHO cells ectopically expressing reqCD154 elicited superoxide production in monocyte-derived macrophages (MDM). Collectively, our results indicate that regulation of CD154 expression plays a role in innate host defenses.

Original language	English
Pages (from-to)	40-44
Number of pages	5
Journal	Veterinary Immunology and Immunopathology
Volume	180
DOIs	https://doi.org/10.1016/j.vetimm.2016.08.001
State	Published - Nov 1 2016

Bibliographical note

Publisher Copyright:
© 2016 Elsevier B.V.

Funding

We acknowledge support from the National Institutes of Health; National Heart, Lung and Blood Institute ( 1R15HL103488-01 ). We thank Ms. Andrea Dorschner, veterinary technician, for assistance in obtaining samples.

Funders	Funder number
National Institutes of Health (NIH)
National Heart, Lung, and Blood Institute Family Blood Pressure Program	R15HL103488

Keywords

CD154
Equine
Reactive intermediates

ASJC Scopus subject areas

Immunology
General Veterinary

Access to Document

10.1016/j.vetimm.2016.08.001

Cite this

Sponseller, B. A., Clark, S. K., Gilbertie, J., Wong, D. M., Hepworth, K., Wiechert, S., Chandramani-Shivalingappa, P., Sponseller, B. T., Alcott, C. J., Bellaire, B., Petersen, A. C., & Jones, D. E. (2016). Macrophage effector responses of horses are influenced by expression of CD154. Veterinary Immunology and Immunopathology, 180, 40-44. https://doi.org/10.1016/j.vetimm.2016.08.001

@article{38a1bcc2d5bc475286d115be821e3b66,

title = "Macrophage effector responses of horses are influenced by expression of CD154",

abstract = "Reactive intermediates contribute to innate immunity by providing phagocytes with a mechanism of defense against bacteria, viruses and parasites. To better characterize the role of CD154 in the production of reactive intermediates, we cloned and expressed recombinant equine CD154 (reqCD154) in Chinese Hamster Ovary (CHO). In co-culture experiments, CHO cells ectopically expressing reqCD154 elicited superoxide production in monocyte-derived macrophages (MDM). Collectively, our results indicate that regulation of CD154 expression plays a role in innate host defenses.",

keywords = "CD154, Equine, Reactive intermediates",

author = "Sponseller, \{Brett A.\} and Clark, \{Sandra K.\} and Jessica Gilbertie and Wong, \{David M.\} and Kate Hepworth and Sarah Wiechert and Prashanth Chandramani-Shivalingappa and Sponseller, \{Beatrice T.\} and Alcott, \{Cody J.\} and Bryan Bellaire and Petersen, \{Andrew C.\} and Jones, \{Douglas E.\}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = nov,

day = "1",

doi = "10.1016/j.vetimm.2016.08.001",

language = "English",

volume = "180",

pages = "40--44",

}

TY - JOUR

T1 - Macrophage effector responses of horses are influenced by expression of CD154

AU - Sponseller, Brett A.

AU - Clark, Sandra K.

AU - Gilbertie, Jessica

AU - Wong, David M.

AU - Hepworth, Kate

AU - Wiechert, Sarah

AU - Chandramani-Shivalingappa, Prashanth

AU - Sponseller, Beatrice T.

AU - Alcott, Cody J.

AU - Bellaire, Bryan

AU - Petersen, Andrew C.

AU - Jones, Douglas E.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Reactive intermediates contribute to innate immunity by providing phagocytes with a mechanism of defense against bacteria, viruses and parasites. To better characterize the role of CD154 in the production of reactive intermediates, we cloned and expressed recombinant equine CD154 (reqCD154) in Chinese Hamster Ovary (CHO). In co-culture experiments, CHO cells ectopically expressing reqCD154 elicited superoxide production in monocyte-derived macrophages (MDM). Collectively, our results indicate that regulation of CD154 expression plays a role in innate host defenses.

AB - Reactive intermediates contribute to innate immunity by providing phagocytes with a mechanism of defense against bacteria, viruses and parasites. To better characterize the role of CD154 in the production of reactive intermediates, we cloned and expressed recombinant equine CD154 (reqCD154) in Chinese Hamster Ovary (CHO). In co-culture experiments, CHO cells ectopically expressing reqCD154 elicited superoxide production in monocyte-derived macrophages (MDM). Collectively, our results indicate that regulation of CD154 expression plays a role in innate host defenses.

KW - CD154

KW - Equine

KW - Reactive intermediates

UR - http://www.scopus.com/inward/record.url?scp=84986538724&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986538724&partnerID=8YFLogxK

U2 - 10.1016/j.vetimm.2016.08.001

DO - 10.1016/j.vetimm.2016.08.001

M3 - Article

C2 - 27692094

AN - SCOPUS:84986538724

SN - 0165-2427

VL - 180

SP - 40

EP - 44

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

ER -

Macrophage effector responses of horses are influenced by expression of CD154

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this