Maize VIVIPAROUS1 Interacts with ABA INSENSITIVE5 to Regulate GALACTINOL SYNTHASE2 Expression Controlling Seed Raffinose Accumulation

Yumin Zhang; Qichao Sun; Chunxia Zhang; Guanglong Hao; Chunmei Wang; Lynnette M.A. Dirk; A. Bruce Downie; Tianyong Zhao

doi:10.1021/acs.jafc.9b00322

Maize VIVIPAROUS1 Interacts with ABA INSENSITIVE5 to Regulate GALACTINOL SYNTHASE2 Expression Controlling Seed Raffinose Accumulation

Yumin Zhang, Qichao Sun, Chunxia Zhang, Guanglong Hao, Chunmei Wang, Lynnette M.A. Dirk, A. Bruce Downie, Tianyong Zhao

Horticulture

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Raffinose, an oligosaccharide found in many seeds, plays an important role in seed vigor; however, the regulatory mechanism governing raffinose biosynthesis remains unclear. We report here that maize W22 wild type (WT) seeds, but not W22 viviparous1 (zmvp1) mutant seeds, start accumulating galactinol and raffinose 28 days after pollination (DAP). Transcriptome analysis of the zmvp1 embryo showed that the expression of GALACTINOL SYNTHASE2 (GOLS2) was down-regulated relative to WT. Further experiments showed that the expression of ZmGOLS2 was up-regulated by ZmABI5 but not by ZmVP1, and it was further increased by the coexpression of ZmABI5 and ZmVP1 in maize protoplasts. ZmABI5 interacted with ZmVP1, while ZmABI5, but not ZmVP1, directly binds to the ZmGOLS2 promoter. Together, all of the findings suggest that ZmVP1 interacts with ZmABI5 and regulates ZmGOLS2 expression and raffinose accumulation in maize seeds.

Original language	English
Pages (from-to)	4214-4223
Number of pages	10
Journal	Journal of Agricultural and Food Chemistry
Volume	67
Issue number	15
DOIs	https://doi.org/10.1021/acs.jafc.9b00322
State	Published - Apr 17 2019

Bibliographical note

Funding Information:
*E-mail: tzhao2@nwafu.edu.cn. Tel: 86-13324517154. ORCID Tianyong Zhao: 0000-0002-1278-2842 Funding Research funding by the National Key Research and Development Plan (2018YFD0100901) and the NSFC (31671776) was awarded to T.Z. For LMAD and ABD, this work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1002299. Notes The authors declare no competing financial interest.

Publisher Copyright:
© 2019 American Chemical Society.

Keywords

ABI5
GALACTINOL SYNTHASE
maize
raffinose biosynthesis
seeds
viviparous

ASJC Scopus subject areas

Chemistry (all)
Agricultural and Biological Sciences (all)

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10.1021/acs.jafc.9b00322

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@article{5056f5f9ffba4765a95c3b1af9bc45a3,

title = "Maize VIVIPAROUS1 Interacts with ABA INSENSITIVE5 to Regulate GALACTINOL SYNTHASE2 Expression Controlling Seed Raffinose Accumulation",

abstract = "Raffinose, an oligosaccharide found in many seeds, plays an important role in seed vigor; however, the regulatory mechanism governing raffinose biosynthesis remains unclear. We report here that maize W22 wild type (WT) seeds, but not W22 viviparous1 (zmvp1) mutant seeds, start accumulating galactinol and raffinose 28 days after pollination (DAP). Transcriptome analysis of the zmvp1 embryo showed that the expression of GALACTINOL SYNTHASE2 (GOLS2) was down-regulated relative to WT. Further experiments showed that the expression of ZmGOLS2 was up-regulated by ZmABI5 but not by ZmVP1, and it was further increased by the coexpression of ZmABI5 and ZmVP1 in maize protoplasts. ZmABI5 interacted with ZmVP1, while ZmABI5, but not ZmVP1, directly binds to the ZmGOLS2 promoter. Together, all of the findings suggest that ZmVP1 interacts with ZmABI5 and regulates ZmGOLS2 expression and raffinose accumulation in maize seeds.",

keywords = "ABI5, GALACTINOL SYNTHASE, maize, raffinose biosynthesis, seeds, viviparous",

author = "Yumin Zhang and Qichao Sun and Chunxia Zhang and Guanglong Hao and Chunmei Wang and Dirk, {Lynnette M.A.} and Downie, {A. Bruce} and Tianyong Zhao",

note = "Funding Information: *E-mail: tzhao2@nwafu.edu.cn. Tel: 86-13324517154. ORCID Tianyong Zhao: 0000-0002-1278-2842 Funding Research funding by the National Key Research and Development Plan (2018YFD0100901) and the NSFC (31671776) was awarded to T.Z. For LMAD and ABD, this work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1002299. Notes The authors declare no competing financial interest. Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

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doi = "10.1021/acs.jafc.9b00322",

language = "English",

volume = "67",

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T1 - Maize VIVIPAROUS1 Interacts with ABA INSENSITIVE5 to Regulate GALACTINOL SYNTHASE2 Expression Controlling Seed Raffinose Accumulation

AU - Zhang, Yumin

AU - Sun, Qichao

AU - Zhang, Chunxia

AU - Hao, Guanglong

AU - Wang, Chunmei

AU - Dirk, Lynnette M.A.

AU - Downie, A. Bruce

AU - Zhao, Tianyong

N1 - Funding Information: *E-mail: tzhao2@nwafu.edu.cn. Tel: 86-13324517154. ORCID Tianyong Zhao: 0000-0002-1278-2842 Funding Research funding by the National Key Research and Development Plan (2018YFD0100901) and the NSFC (31671776) was awarded to T.Z. For LMAD and ABD, this work was supported by the USDA National Institute of Food and Agriculture, Hatch project 1002299. Notes The authors declare no competing financial interest. Publisher Copyright: © 2019 American Chemical Society.

PY - 2019/4/17

Y1 - 2019/4/17

N2 - Raffinose, an oligosaccharide found in many seeds, plays an important role in seed vigor; however, the regulatory mechanism governing raffinose biosynthesis remains unclear. We report here that maize W22 wild type (WT) seeds, but not W22 viviparous1 (zmvp1) mutant seeds, start accumulating galactinol and raffinose 28 days after pollination (DAP). Transcriptome analysis of the zmvp1 embryo showed that the expression of GALACTINOL SYNTHASE2 (GOLS2) was down-regulated relative to WT. Further experiments showed that the expression of ZmGOLS2 was up-regulated by ZmABI5 but not by ZmVP1, and it was further increased by the coexpression of ZmABI5 and ZmVP1 in maize protoplasts. ZmABI5 interacted with ZmVP1, while ZmABI5, but not ZmVP1, directly binds to the ZmGOLS2 promoter. Together, all of the findings suggest that ZmVP1 interacts with ZmABI5 and regulates ZmGOLS2 expression and raffinose accumulation in maize seeds.

AB - Raffinose, an oligosaccharide found in many seeds, plays an important role in seed vigor; however, the regulatory mechanism governing raffinose biosynthesis remains unclear. We report here that maize W22 wild type (WT) seeds, but not W22 viviparous1 (zmvp1) mutant seeds, start accumulating galactinol and raffinose 28 days after pollination (DAP). Transcriptome analysis of the zmvp1 embryo showed that the expression of GALACTINOL SYNTHASE2 (GOLS2) was down-regulated relative to WT. Further experiments showed that the expression of ZmGOLS2 was up-regulated by ZmABI5 but not by ZmVP1, and it was further increased by the coexpression of ZmABI5 and ZmVP1 in maize protoplasts. ZmABI5 interacted with ZmVP1, while ZmABI5, but not ZmVP1, directly binds to the ZmGOLS2 promoter. Together, all of the findings suggest that ZmVP1 interacts with ZmABI5 and regulates ZmGOLS2 expression and raffinose accumulation in maize seeds.

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KW - viviparous

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Maize VIVIPAROUS1 Interacts with ABA INSENSITIVE5 to Regulate GALACTINOL SYNTHASE2 Expression Controlling Seed Raffinose Accumulation

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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