Extracts of a Pseudomonas species (Shaw strain MA) contain an inducible malate cleavage activity: Malate + ATP + CoA /ag Glyxylate + AcCoA + ADP + Pi. The stoichiometry of the forward reaction was shown to be one mole of glyoxylate produced per mole of ATP hydrolyzed, while in the reverse reaction all three products; malate, ATP, and CoA, were formed in equivalent amounts. The forward reaction shows a broad pH optima of 7-8, while the reverse reaction has a fairly sharp optima of pH 8.2. It is suggested that this enzyme plays a key role in the metabolism of reduced one-carbon compounds by Pseudomonas species-MA.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Aug 7 1972|
Bibliographical noteFunding Information:
in part by Grant No. I 391 from The Robert A. Texas, and Grant No. AM 13443 from the NIAMD to
Copyright 2014 Elsevier B.V., All rights reserved.
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology