Abstract
Extracts of a Pseudomonas species (Shaw strain MA) contain an inducible malate cleavage activity: Malate + ATP + CoA /ag Glyxylate + AcCoA + ADP + Pi. The stoichiometry of the forward reaction was shown to be one mole of glyoxylate produced per mole of ATP hydrolyzed, while in the reverse reaction all three products; malate, ATP, and CoA, were formed in equivalent amounts. The forward reaction shows a broad pH optima of 7-8, while the reverse reaction has a fairly sharp optima of pH 8.2. It is suggested that this enzyme plays a key role in the metabolism of reduced one-carbon compounds by Pseudomonas species-MA.
| Original language | English |
|---|---|
| Pages (from-to) | 712-719 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 48 |
| Issue number | 3 |
| DOIs | |
| State | Published - Aug 7 1972 |
Bibliographical note
Funding Information:in part by Grant No. I 391 from The Robert A. Texas, and Grant No. AM 13443 from the NIAMD to
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology