Malate cleavage reaction in Pseudomonas species, (Shaw strain MA)

Louis B. Hersh, Edward Bellion

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Extracts of a Pseudomonas species (Shaw strain MA) contain an inducible malate cleavage activity: Malate + ATP + CoA /ag Glyxylate + AcCoA + ADP + Pi. The stoichiometry of the forward reaction was shown to be one mole of glyoxylate produced per mole of ATP hydrolyzed, while in the reverse reaction all three products; malate, ATP, and CoA, were formed in equivalent amounts. The forward reaction shows a broad pH optima of 7-8, while the reverse reaction has a fairly sharp optima of pH 8.2. It is suggested that this enzyme plays a key role in the metabolism of reduced one-carbon compounds by Pseudomonas species-MA.

Original languageEnglish
Pages (from-to)712-719
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Aug 7 1972

Bibliographical note

Funding Information:
in part by Grant No. I 391 from The Robert A. Texas, and Grant No. AM 13443 from the NIAMD to

Copyright 2014 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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