Manganese superoxide dismutase expression in human cancer cells: A possible role of mRNA processing

Daret K. St Clair, Larry W. Oberley

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The level of manganese superoxide dismutase (MnSOD) as determined by an immunorcactive assay was reduced in human cancer cells. The reduced amount of immunoreactive MnSOD in these cells was observed regardless of the growth state of the cells. The decrease in the enzyme protein was associated with a decrease in the mature form of MnSOD transcript as determined by Northern Analysis. The decreased amount of the mature form of MnSOD transcript was accompanied by an increase in the amount of the partially processed form of MnSOD transcript. These results suggest that RNA processing or translation of MnSOD mRNA may be responsible for the decreased amount of MnSOD activity in human tumor cells.

Original languageEnglish
Pages (from-to)771-778
Number of pages8
JournalFree Radical Research
Volume13
Issue number1
DOIs
StatePublished - 1991

Bibliographical note

Funding Information:
This work was supported in part by a grant from the Forsyth Cancer Service, Inc., and NIH grants RR-0504. CA 41267. CA 12197-17 and CA 49797. The authors thank Pamela D. Cregger for preparation of this manuscript.

Keywords

  • Human cancer
  • MRNA processing
  • MnSOD
  • Northern blot
  • Western blot

ASJC Scopus subject areas

  • Biochemistry

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