Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts

Larry W. Oberley; Michael L. McCormick; Elaine Sierra-Rivera; Daret Kasemset-St. Clair

doi:10.1016/0891-5849(89)90083-X

Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts

Larry W. Oberley, Michael L. McCormick, Elaine Sierra-Rivera, Daret Kasemset-St. Clair

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

The manganese superoxide dismutase (MnSOD) activity of WI38 human embryonic lung fibroblasts and SV40-transformed WI38 cells was measured. Under various growth conditions, the transformed cells always had lower MnSOD activity than their normal cell counterparts. The activity of MnSOD changes greatly with the growth conditions in the WI38 cells, while the MnSOD activity in the tumor cells remained more constant. The amount of immunoreactive MnSOD was measured by Western blotting. In all cases studied, the amount of immunoreactive MnSOD was lower in the transformed cells than in normal cells.

Original language	English
Pages (from-to)	379-384
Number of pages	6
Journal	Free Radical Biology and Medicine
Volume	6
Issue number	4
DOIs	https://doi.org/10.1016/0891-5849(89)90083-X
State	Published - 1989

Bibliographical note

Funding Information:
Acknowledgements--This research was supported by NIH grants IR01-CA41267 and 5T32-CA09125. The authors would like to thank James Elwell, Lisa Ridnour, Tom Deahl, and Gregg Cohen for their excellent technical assistance.

Keywords

Free radical
Human fibroblasts
Manganese superoxide dismutase
SV40
WI38
Western blotting

ASJC Scopus subject areas

Biochemistry
Physiology (medical)

Access to Document

10.1016/0891-5849(89)90083-X

Cite this

@article{b53d27483cab4fd186bda451878488eb,

title = "Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts",

abstract = "The manganese superoxide dismutase (MnSOD) activity of WI38 human embryonic lung fibroblasts and SV40-transformed WI38 cells was measured. Under various growth conditions, the transformed cells always had lower MnSOD activity than their normal cell counterparts. The activity of MnSOD changes greatly with the growth conditions in the WI38 cells, while the MnSOD activity in the tumor cells remained more constant. The amount of immunoreactive MnSOD was measured by Western blotting. In all cases studied, the amount of immunoreactive MnSOD was lower in the transformed cells than in normal cells.",

keywords = "Free radical, Human fibroblasts, Manganese superoxide dismutase, SV40, WI38, Western blotting",

author = "Oberley, {Larry W.} and McCormick, {Michael L.} and Elaine Sierra-Rivera and {Kasemset-St. Clair}, Daret",

note = "Funding Information: Acknowledgements--This research was supported by NIH grants IR01-CA41267 and 5T32-CA09125. The authors would like to thank James Elwell, Lisa Ridnour, Tom Deahl, and Gregg Cohen for their excellent technical assistance.",

year = "1989",

doi = "10.1016/0891-5849(89)90083-X",

language = "English",

volume = "6",

pages = "379--384",

number = "4",

}

TY - JOUR

T1 - Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts

AU - Oberley, Larry W.

AU - McCormick, Michael L.

AU - Sierra-Rivera, Elaine

AU - Kasemset-St. Clair, Daret

N1 - Funding Information: Acknowledgements--This research was supported by NIH grants IR01-CA41267 and 5T32-CA09125. The authors would like to thank James Elwell, Lisa Ridnour, Tom Deahl, and Gregg Cohen for their excellent technical assistance.

PY - 1989

Y1 - 1989

N2 - The manganese superoxide dismutase (MnSOD) activity of WI38 human embryonic lung fibroblasts and SV40-transformed WI38 cells was measured. Under various growth conditions, the transformed cells always had lower MnSOD activity than their normal cell counterparts. The activity of MnSOD changes greatly with the growth conditions in the WI38 cells, while the MnSOD activity in the tumor cells remained more constant. The amount of immunoreactive MnSOD was measured by Western blotting. In all cases studied, the amount of immunoreactive MnSOD was lower in the transformed cells than in normal cells.

AB - The manganese superoxide dismutase (MnSOD) activity of WI38 human embryonic lung fibroblasts and SV40-transformed WI38 cells was measured. Under various growth conditions, the transformed cells always had lower MnSOD activity than their normal cell counterparts. The activity of MnSOD changes greatly with the growth conditions in the WI38 cells, while the MnSOD activity in the tumor cells remained more constant. The amount of immunoreactive MnSOD was measured by Western blotting. In all cases studied, the amount of immunoreactive MnSOD was lower in the transformed cells than in normal cells.

KW - Free radical

KW - Human fibroblasts

KW - Manganese superoxide dismutase

KW - SV40

KW - WI38

KW - Western blotting

UR - http://www.scopus.com/inward/record.url?scp=0024596776&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024596776&partnerID=8YFLogxK

U2 - 10.1016/0891-5849(89)90083-X

DO - 10.1016/0891-5849(89)90083-X

M3 - Article

C2 - 2540070

AN - SCOPUS:0024596776

SN - 0891-5849

VL - 6

SP - 379

EP - 384

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

IS - 4

ER -

Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this