Manganese superoxide dismutase in normal and transformed human embryonic lung fibroblasts

Larry W. Oberley, Michael L. McCormick, Elaine Sierra-Rivera, Daret Kasemset-St. Clair

Research output: Contribution to journalArticlepeer-review

84 Scopus citations


The manganese superoxide dismutase (MnSOD) activity of WI38 human embryonic lung fibroblasts and SV40-transformed WI38 cells was measured. Under various growth conditions, the transformed cells always had lower MnSOD activity than their normal cell counterparts. The activity of MnSOD changes greatly with the growth conditions in the WI38 cells, while the MnSOD activity in the tumor cells remained more constant. The amount of immunoreactive MnSOD was measured by Western blotting. In all cases studied, the amount of immunoreactive MnSOD was lower in the transformed cells than in normal cells.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalFree Radical Biology and Medicine
Issue number4
StatePublished - 1989

Bibliographical note

Funding Information:
Acknowledgements--This research was supported by NIH grants IR01-CA41267 and 5T32-CA09125. The authors would like to thank James Elwell, Lisa Ridnour, Tom Deahl, and Gregg Cohen for their excellent technical assistance.


  • Free radical
  • Human fibroblasts
  • Manganese superoxide dismutase
  • SV40
  • WI38
  • Western blotting

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


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