Mass spectrometric evidence for aldehyde adduction in carboxymyoglobin

P. Joseph, S. P. Suman, R. A. Mancini, C. M. Beach

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Lipid oxidation generates secondary oxidation products, which compromise myoglobin (Mb) redox stability. Although lipid oxidation-induced discoloration in carboxymyoglobin (COMb) is documented, the molecular basis for interactions between COMb and lipid oxidation products has not been investigated. Our objective was to characterize the adduction of 4-hydroxy-2-nonenal (HNE), a reactive lipid oxidation product, with COMb, utilizing mass spectrometry. Equine COMb and equine OxyMb (0.15 mM) were incubated with HNE (1.0 mM) at pH 7.4, 37 °C (physiological condition) for 6 h, and at pH 5.6, 4 °C (typical meat storage condition) for 7 days. The samples were analyzed in Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS). MS spectra revealed that HNE formed mono-, di-, and tri-adducts with COMb at physiological conditions, whereas mono-, di-, tri-, and tetra-adducts were detected in OxyMb. This observation suggested a lower reactivity of COMb towards HNE at physiological conditions, compared to OxyMb. In contrast, at meat storage conditions, HNE formed mono- and di-adducts with both COMb and OxyMb, thus revealing a similar trend for aldehyde adduction in the cherry-red colored Mb redox forms. The present study is the first to report HNE adduction in COMb, and proteomic investigations are underway to determine the sites of HNE adduction in COMb.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalMeat Science
Issue number3
StatePublished - Nov 2009

Bibliographical note

Funding Information:
This work was supported by funds from the Kentucky Agricultural Experiment Station, University of Kentucky. The mass spectrometric analysis was performed at the University of Kentucky’s Center for Structural Biology Protein Core Facility, supported in part by funds from the National Institute of Health’s National Center for Research Resources grant P20 RR020171.


  • 4-Hydroxy-2-nonenal
  • Carbon monoxide
  • Carboxymyoglobin
  • Lipid oxidation
  • Mass spectrometry
  • Modified atmosphere packaging

ASJC Scopus subject areas

  • Food Science


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