Abstract
Mass spectrometry-based investigation of clinical samples enables the high-throughput identification of protein biomarkers. We provide an overview of mass spectrometry-based proteomic techniques that are applicable to the investigation of clinical samples. We address sample collection, protein extraction and fractionation, mass spectrometry modalities, and quantitative proteomics. Finally, we examine the limitations and further potential of such technologies. Liquid chromatography fractionation coupled with tandem mass spectrometry is well suited to handle mixtures of hundreds or thousands of proteins. Mass spectrometry-based proteome elucidation can reveal potential biomarkers and aid in the development of hypotheses for downstream investigation of the molecular mechanisms of disease.
Original language | English |
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Pages (from-to) | 59-73 |
Number of pages | 15 |
Journal | Yale Journal of Biology and Medicine |
Volume | 85 |
Issue number | 1 |
State | Published - Mar 2012 |
Keywords
- Biomarkers
- Chronic pancreatitis
- Mass spectrometry
- Pancreas
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology