Mass spectrometry-based proteomics for translational research: A technical overview

Joao A. Paulo, Vivek Kadiyala, Peter A. Banks, Hanno Steen, Darwin L. Conwell

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Mass spectrometry-based investigation of clinical samples enables the high-throughput identification of protein biomarkers. We provide an overview of mass spectrometry-based proteomic techniques that are applicable to the investigation of clinical samples. We address sample collection, protein extraction and fractionation, mass spectrometry modalities, and quantitative proteomics. Finally, we examine the limitations and further potential of such technologies. Liquid chromatography fractionation coupled with tandem mass spectrometry is well suited to handle mixtures of hundreds or thousands of proteins. Mass spectrometry-based proteome elucidation can reveal potential biomarkers and aid in the development of hypotheses for downstream investigation of the molecular mechanisms of disease.

Original languageEnglish
Pages (from-to)59-73
Number of pages15
JournalYale Journal of Biology and Medicine
Volume85
Issue number1
StatePublished - Mar 2012

Keywords

  • Biomarkers
  • Chronic pancreatitis
  • Mass spectrometry
  • Pancreas

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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