Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research

Joao A. Paulo, Linda S. Lee, Bechien Wu, Peter A. Banks, Hanno Steen, Darwin L. Conwell

Research output: Contribution to journalReview articlepeer-review

18 Scopus citations

Abstract

MS-based investigation of pancreatic fluid enables the high-throughput identification of proteins present in the pancreatic secretome. Pancreatic fluid is a complex admixture of digestive, inflammatory, and other proteins secreted by the pancreas into the duodenum, and thus is amenable to MS-based proteomic analysis. Recent advances in endoscopic techniques, in particular the endoscopic pancreatic function test (ePFT), have improved the collection methodology of pancreatic fluid for proteomic analysis. Here, we provide an overview of MS-based proteomic techniques as applied to the study of pancreatic fluid. We address sample collection, protein extraction, MS sample preparation and analysis, and bioinformatic approaches, and summarize current MS-based investigations of pancreatic fluid. We then examine the limitations and the future potential of such technologies in the investigation of pancreatic disease. We conclude that pancreatic fluid represents a rich reservoir of potential biomarkers and that the study of the molecular mechanisms of chronic pancreatitis may benefit substantially from MS-based proteomics.

Original languageEnglish
Pages (from-to)109-120
Number of pages12
JournalProteomics - Clinical Applications
Volume5
Issue number3-4
DOIs
StatePublished - Apr 2011

Keywords

  • Chronic pancreatitis
  • EPFT
  • Mass spectrometry
  • Pancreatic juice

ASJC Scopus subject areas

  • Clinical Biochemistry

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