TY - JOUR
T1 - Mass spectrometry-based proteomics of endoscopically collected pancreatic fluid in chronic pancreatitis research
AU - Paulo, Joao A.
AU - Lee, Linda S.
AU - Wu, Bechien
AU - Banks, Peter A.
AU - Steen, Hanno
AU - Conwell, Darwin L.
PY - 2011/4
Y1 - 2011/4
N2 - MS-based investigation of pancreatic fluid enables the high-throughput identification of proteins present in the pancreatic secretome. Pancreatic fluid is a complex admixture of digestive, inflammatory, and other proteins secreted by the pancreas into the duodenum, and thus is amenable to MS-based proteomic analysis. Recent advances in endoscopic techniques, in particular the endoscopic pancreatic function test (ePFT), have improved the collection methodology of pancreatic fluid for proteomic analysis. Here, we provide an overview of MS-based proteomic techniques as applied to the study of pancreatic fluid. We address sample collection, protein extraction, MS sample preparation and analysis, and bioinformatic approaches, and summarize current MS-based investigations of pancreatic fluid. We then examine the limitations and the future potential of such technologies in the investigation of pancreatic disease. We conclude that pancreatic fluid represents a rich reservoir of potential biomarkers and that the study of the molecular mechanisms of chronic pancreatitis may benefit substantially from MS-based proteomics.
AB - MS-based investigation of pancreatic fluid enables the high-throughput identification of proteins present in the pancreatic secretome. Pancreatic fluid is a complex admixture of digestive, inflammatory, and other proteins secreted by the pancreas into the duodenum, and thus is amenable to MS-based proteomic analysis. Recent advances in endoscopic techniques, in particular the endoscopic pancreatic function test (ePFT), have improved the collection methodology of pancreatic fluid for proteomic analysis. Here, we provide an overview of MS-based proteomic techniques as applied to the study of pancreatic fluid. We address sample collection, protein extraction, MS sample preparation and analysis, and bioinformatic approaches, and summarize current MS-based investigations of pancreatic fluid. We then examine the limitations and the future potential of such technologies in the investigation of pancreatic disease. We conclude that pancreatic fluid represents a rich reservoir of potential biomarkers and that the study of the molecular mechanisms of chronic pancreatitis may benefit substantially from MS-based proteomics.
KW - Chronic pancreatitis
KW - EPFT
KW - Mass spectrometry
KW - Pancreatic juice
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UR - http://www.scopus.com/inward/citedby.url?scp=79953123184&partnerID=8YFLogxK
U2 - 10.1002/prca.201000098
DO - 10.1002/prca.201000098
M3 - Review article
C2 - 21360826
AN - SCOPUS:79953123184
SN - 1862-8346
VL - 5
SP - 109
EP - 120
JO - Proteomics - Clinical Applications
JF - Proteomics - Clinical Applications
IS - 3-4
ER -