Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Tara R. Hawkinson; Ramon C. Sun

doi:10.1007/978-1-0716-2030-4_15

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Tara R. Hawkinson, Ramon C. Sun

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Scopus citations

Abstract

Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	215-228
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-0716-2030-4_15
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2437
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
R.C.S. is supported through NIH R01 grant AG066653-01; St. Baldrick’s career development award; Rally foundation independent investigator grant; V-scholar foundation award; and University of Kentucky College of Medicine and Markey Cancer Center start-up funds. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH-funded Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558.

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

Alzheimer’s disease
FFPE tissue
Glycogen
Glycogen chain length
Isoamylase
MALDI-MSI

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-0716-2030-4_15

Cite this

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title = "Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ",

abstract = "Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.",

keywords = "Alzheimer{\textquoteright}s disease, FFPE tissue, Glycogen, Glycogen chain length, Isoamylase, MALDI-MSI",

author = "Hawkinson, {Tara R.} and Sun, {Ramon C.}",

note = "Funding Information: R.C.S. is supported through NIH R01 grant AG066653-01; St. Baldrick{\textquoteright}s career development award; Rally foundation independent investigator grant; V-scholar foundation award; and University of Kentucky College of Medicine and Markey Cancer Center start-up funds. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH-funded Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2030-4_15",

language = "English",

series = "Methods in Molecular Biology",

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AU - Sun, Ramon C.

N1 - Funding Information: R.C.S. is supported through NIH R01 grant AG066653-01; St. Baldrick’s career development award; Rally foundation independent investigator grant; V-scholar foundation award; and University of Kentucky College of Medicine and Markey Cancer Center start-up funds. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH-funded Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558. Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

AB - Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

KW - Alzheimer’s disease

KW - FFPE tissue

KW - Glycogen

KW - Glycogen chain length

KW - Isoamylase

KW - MALDI-MSI

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

ER -

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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