Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Tara R. Hawkinson; Ramon C. Sun

doi:10.1007/978-1-0716-2030-4_15

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Tara R. Hawkinson
, Ramon C. Sun

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

15 Scopus citations

Abstract

Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	215-228
Number of pages	14
DOIs	https://doi.org/10.1007/978-1-0716-2030-4_15
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2437
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Funding

R.C.S. is supported through NIH R01 grant AG066653-01; St. Baldrick’s career development award; Rally foundation independent investigator grant; V-scholar foundation award; and University of Kentucky College of Medicine and Markey Cancer Center start-up funds. This research was also supported by funding from the University of Kentucky Markey Cancer Center and the NIH-funded Biospecimen Procurement & Translational Pathology Shared Resource Facility of the University of Kentucky Markey Cancer Center P30CA177558.

Funders	Funder number
NIH-funded University of Kentucky Center for Cancer and Metabolism	P30CA177558
Departments of Urology, Pathology, The Markey Cancer Center and Department of Toxicology, University of Kentucky College of Medicine
National Institutes of Health (NIH)
National Institute on Aging	R01AG066653
National Institute on Aging
University of Kentucky Markey Cancer Center

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Alzheimer’s disease
FFPE tissue
Glycogen
Glycogen chain length
Isoamylase
MALDI-MSI

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-2030-4_15

Cite this

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title = "Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ",

abstract = "Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.",

keywords = "Alzheimer{\textquoteright}s disease, FFPE tissue, Glycogen, Glycogen chain length, Isoamylase, MALDI-MSI",

author = "Hawkinson, \{Tara R.\} and Sun, \{Ramon C.\}",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2022",

doi = "10.1007/978-1-0716-2030-4\_15",

language = "English",

series = "Methods in Molecular Biology",

pages = "215--228",

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T1 - Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

AU - Hawkinson, Tara R.

AU - Sun, Ramon C.

PY - 2022

Y1 - 2022

N2 - Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

AB - Histopathological detection and quantitation of glycogen in situ are important for the assessment of glycogen storage diseases and different types of cancer. The current standard method for defining the regionality of glycogen rely almost exclusively on Periodic Acid-Schiff (PAS) staining, a workflow that lacks specificity and sensitivity. Herein, we describe a new and much improved workflow to detect microenvironmental glycogen in situ using enzyme-assisted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI). This method provides superior sensitivity and can elucidate the molecular features of glycogen structure, with 50 μm spatial resolution for a next-generation histopathological assessment of glycogen.

KW - Alzheimer’s disease

KW - FFPE tissue

KW - Glycogen

KW - Glycogen chain length

KW - Isoamylase

KW - MALDI-MSI

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UR - https://www.scopus.com/pages/publications/85121569422#tab=citedBy

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DO - 10.1007/978-1-0716-2030-4_15

M3 - Chapter

C2 - 34902151

AN - SCOPUS:85121569422

T3 - Methods in Molecular Biology

SP - 215

EP - 228

BT - Methods in Molecular Biology

ER -

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging of Glycogen In Situ

Abstract

Publication series

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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Cite this