Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors that regulate transcription of target genes. Since attempts have been made to correlate the ingestion of high-fat diets, itself a peroxisome proliferator, with the occurrence of breast cancer, we set about to determine if human breast cancer cells contained a functional PPAR. In this report we demonstrate the presence of an mRNA in two breast cancer cell lines (MCF-7 and T47D) which is specifically recognized by a mouse PPARγ2 probe. Furthermore, in gel shift assays a consensus PPAR response element (PPRE) was specifically bound by nuclear extracts from MCF-7 cells and was further retarded by antibodies raised to mouse PPARγ. Finally, when transfected with a PPRE-luciferase transcriptional reporter construct, transcription was increased in response to activators of PPAR and its dimmeric partner the retinoic acid X receptor (RXR). These data indicate that peroxisomal proliferators are capable of mediating transcription in human breast cells and suggest the possibility of a physiological role in the breast.
|Number of pages||7|
|Journal||Molecular and Cellular Endocrinology|
|State||Published - 1997|
Bibliographical noteFunding Information:
We would like to acknowledge Evan Simpson, M. Dod Michael and Ron Evans for plasmids used in these studies. We also would like to thank Douglas Stocco, M. Dod Michael and Anna Lauber-Biason for critical discussion of this work. This work was supported by the Greenville Hospital System/Clemson University Cooperative Research and Education program.
- Human breast cancer
- MCF-7 cells
- Peroxisomal proliferator
ASJC Scopus subject areas
- Molecular Biology