MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response

Michael W. Kilgore, Patricia L. Tate, Sudha Rai, Eiichi Sengoku, Thomas M. Price

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors that regulate transcription of target genes. Since attempts have been made to correlate the ingestion of high-fat diets, itself a peroxisome proliferator, with the occurrence of breast cancer, we set about to determine if human breast cancer cells contained a functional PPAR. In this report we demonstrate the presence of an mRNA in two breast cancer cell lines (MCF-7 and T47D) which is specifically recognized by a mouse PPARγ2 probe. Furthermore, in gel shift assays a consensus PPAR response element (PPRE) was specifically bound by nuclear extracts from MCF-7 cells and was further retarded by antibodies raised to mouse PPARγ. Finally, when transfected with a PPRE-luciferase transcriptional reporter construct, transcription was increased in response to activators of PPAR and its dimmeric partner the retinoic acid X receptor (RXR). These data indicate that peroxisomal proliferators are capable of mediating transcription in human breast cells and suggest the possibility of a physiological role in the breast.

Original languageEnglish
Pages (from-to)229-235
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume129
Issue number2
DOIs
StatePublished - 1997

Bibliographical note

Funding Information:
We would like to acknowledge Evan Simpson, M. Dod Michael and Ron Evans for plasmids used in these studies. We also would like to thank Douglas Stocco, M. Dod Michael and Anna Lauber-Biason for critical discussion of this work. This work was supported by the Greenville Hospital System/Clemson University Cooperative Research and Education program.

Keywords

  • Human breast cancer
  • MCF-7 cells
  • PPAR
  • Peroxisomal proliferator

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Fingerprint

Dive into the research topics of 'MCF-7 and T47D human breast cancer cells contain a functional peroxisomal response'. Together they form a unique fingerprint.

Cite this