Measurement of Autotaxin/Lysophospholipase D Activity

Andrew J. Morris; Susan S. Smyth

doi:10.1016/S0076-6879(07)34005-6

Measurement of Autotaxin/Lysophospholipase D Activity

Andrew J. Morris, Susan S. Smyth

Internal Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

14 Scopus citations

Abstract

Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.

Original language	English
Title of host publication	Lipidomics and Bioactive Lipids
Subtitle of host publication	Lipids and Cell Signaling
Editors	Alex Brown
Pages	89-104
Number of pages	16
DOIs	https://doi.org/10.1016/S0076-6879(07)34005-6
State	Published - 2007

Publication series

Name	Methods in Enzymology
Volume	434
ISSN (Print)	0076-6879

Bibliographical note

Funding Information:
Research in the authors' laboratories is supported by grants from the National Institutes of Health.

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/S0076-6879(07)34005-6

Cite this

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title = "Measurement of Autotaxin/Lysophospholipase D Activity",

abstract = "Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.",

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N2 - Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.

AB - Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.

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Measurement of Autotaxin/Lysophospholipase D Activity

Abstract

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