Measurement of Autotaxin/Lysophospholipase D Activity

Andrew J. Morris, Susan S. Smyth

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

14 Scopus citations

Abstract

Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in the blood and other biological fluids at physiologically relevant concentrations. In the cardiovascular system, studies using in vitro and in vivo experimental models indicate that LPA stimulates platelet activation, differentiation and migration of vascular smooth muscle cells, and changes in vascular tone. A growing body of evidence suggests that aberrant production and actions of LPA could play an important role in atherothrombotic disease. Hydrolysis of lysophospholipids by the secreted plasma protein autotaxin/lysophospholipase D (lysoPLD) is a major mechanism for generation of LPA in the blood. This chapter describes methods for determining the activity of recombinant autotaxin/lysoPLD using radiolabeled and fluorogenic substrates.

Original languageEnglish
Title of host publicationLipidomics and Bioactive Lipids
Subtitle of host publicationLipids and Cell Signaling
EditorsAlex Brown
Pages89-104
Number of pages16
DOIs
StatePublished - 2007

Publication series

NameMethods in Enzymology
Volume434
ISSN (Print)0076-6879

Bibliographical note

Funding Information:
Research in the authors' laboratories is supported by grants from the National Institutes of Health.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Measurement of Autotaxin/Lysophospholipase D Activity'. Together they form a unique fingerprint.

Cite this