Measurement of lysophosphatidic acid and sphingosine-1-phosphate by liquid chromatography-coupled electrospray ionization tandem mass spectrometry

Maria P. Kraemer; Suchismita Halder; Susan S. Smyth; Andrew J. Morris

doi:10.1007/7651_2017_55

Measurement of lysophosphatidic acid and sphingosine-1-phosphate by liquid chromatography-coupled electrospray ionization tandem mass spectrometry

Maria P. Kraemer, Suchismita Halder, Susan S. Smyth, Andrew J. Morris

Internal Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Scopus citations

Abstract

Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	31-42
Number of pages	12
DOIs	https://doi.org/10.1007/7651_2017_55
State	Published - 2018

Publication series

Name	Methods in Molecular Biology
Volume	1697
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media New York.

Keywords

Liquid chromatography
Lysophospholipids
Mass spectrometry

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/7651_2017_55

Cite this

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abstract = "Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.",

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Measurement of lysophosphatidic acid and sphingosine-1-phosphate by liquid chromatography-coupled electrospray ionization tandem mass spectrometry. / Kraemer, Maria P.; Halder, Suchismita; Smyth, Susan S. et al.
Methods in Molecular Biology. 2018. p. 31-42 (Methods in Molecular Biology; Vol. 1697).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Morris, Andrew J.

PY - 2018

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KW - Lysophospholipids

KW - Mass spectrometry

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Measurement of lysophosphatidic acid and sphingosine-1-phosphate by liquid chromatography-coupled electrospray ionization tandem mass spectrometry

Abstract

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Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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