Measurement of lysophosphatidic acid and sphingosine-1-phosphate by liquid chromatography-coupled electrospray ionization tandem mass spectrometry

Maria P. Kraemer, Suchismita Halder, Susan S. Smyth, Andrew J. Morris

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations

Abstract

Lysophosphatidic acids and sphingosine-1-phosphate are bioactive lipids that regulate diverse cellular and physiological processes through actions that are largely mediated by cell surface receptors. The roles played by these lipids in multiple disease processes make the enzymes and receptors involved in their synthesis, inactivation, and signaling attractive targets for pharmacological therapies. In this chapter we describe methods for sensitive accurate quantitation of LPA and S1P levels in biological fluids using liquid chromatography-coupled electrospray ionization tandem mass spectrometry.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages31-42
Number of pages12
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1697
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media New York.

Funding

FundersFunder number
U.S. Department of Veterans AffairsI01CX001550

    Keywords

    • Liquid chromatography
    • Lysophospholipids
    • Mass spectrometry

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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