Adhesion of bacteria to oral implant surfaces can lead to oral infections, and the prevention of strong biofilm adherence to implant surfaces can assist in the prevention of these infections like peri-implantitis. In prior studies, single species biofilm adhesion has been quantitatively measured via the laser spallation technique. However, colonizing oral biofilms rarely consists of a single bacteria species. Multiple early colonizer species, including several strains of Streptococci, dominate initial oral biofilm formation. This study aims to characterize the adhesion of a multi-species oral biofilm consisting of S. oralis, S. sanguinis, and S. gordonii on titanium, a common implant material, using the laser spallation technique. Previous work has established these specific Streptococci strains as a multi-species periodontal biofilm model. This study is the first to provide a quantitative adhesion measurement of this multi-species model onto a dental implant surface. First, adhesion strength of the multi-species model is compared to adhesion strength of the single-species streptococci constituents. Fluorescent staining and imaging by fluorescent microscopy are used to identify individual bacteria species within the biofilm. The multi-species biofilm presented in this study provides a more representative model of in vivo early biofilms and provides a more accurate metric for understanding biocompatibility on implant surfaces.
|Title of host publication||Challenges in Mechanics of Time-Dependent Materials and Mechanics of Biological Systems and Materials, Volume 2 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics|
|Editors||Alireza Amirkhizi, Jevan Furmanski, Christian Franck, Karen Kasza, Aaron Forster, Jon Estrada|
|Number of pages||5|
|State||Published - 2023|
|Event||SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2022 - Pittsburgh, United States|
Duration: Jun 13 2022 → Jun 16 2022
|Name||Conference Proceedings of the Society for Experimental Mechanics Series|
|Conference||SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2022|
|Period||6/13/22 → 6/16/22|
Bibliographical noteFunding Information:
Funding This material is based upon work supported by the National Science Foundation CAREER Award grant number 2045853. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.
We gratefully acknowledge NIH Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation (CPRI, P20GM130456) and NIH NIDCR funding (R03DE029547) for completion of these experiments. Funding This material is based upon work supported by the National Science Foundation CAREER Award grant number 2045853. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.
© 2023, The Society for Experimental Mechanics, Inc.
- Dental implants
- Laser spallation
ASJC Scopus subject areas
- Engineering (all)
- Computational Mechanics
- Mechanical Engineering