Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface

M. N. Hessin; J. D. Boyd; M. E. Grady

doi:10.1007/978-3-031-17457-5_11

Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface

M. N. Hessin, J. D. Boyd, M. E. Grady

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Adhesion of bacteria to oral implant surfaces can lead to oral infections, and the prevention of strong biofilm adherence to implant surfaces can assist in the prevention of these infections like peri-implantitis. In prior studies, single species biofilm adhesion has been quantitatively measured via the laser spallation technique. However, colonizing oral biofilms rarely consists of a single bacteria species. Multiple early colonizer species, including several strains of Streptococci, dominate initial oral biofilm formation. This study aims to characterize the adhesion of a multi-species oral biofilm consisting of S. oralis, S. sanguinis, and S. gordonii on titanium, a common implant material, using the laser spallation technique. Previous work has established these specific Streptococci strains as a multi-species periodontal biofilm model. This study is the first to provide a quantitative adhesion measurement of this multi-species model onto a dental implant surface. First, adhesion strength of the multi-species model is compared to adhesion strength of the single-species streptococci constituents. Fluorescent staining and imaging by fluorescent microscopy are used to identify individual bacteria species within the biofilm. The multi-species biofilm presented in this study provides a more representative model of in vivo early biofilms and provides a more accurate metric for understanding biocompatibility on implant surfaces.

Original language	English
Title of host publication	Challenges in Mechanics of Time-Dependent Materials and Mechanics of Biological Systems and Materials, Volume 2 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics
Editors	Alireza Amirkhizi, Jevan Furmanski, Christian Franck, Karen Kasza, Aaron Forster, Jon Estrada
Pages	71-75
Number of pages	5
DOIs	https://doi.org/10.1007/978-3-031-17457-5_11
State	Published - 2023
Event	SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2022 - Pittsburgh, United States Duration: Jun 13 2022 → Jun 16 2022

Publication series

Name	Conference Proceedings of the Society for Experimental Mechanics Series
ISSN (Print)	2191-5644
ISSN (Electronic)	2191-5652

Conference

Conference	SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2022
Country/Territory	United States
City	Pittsburgh
Period	6/13/22 → 6/16/22

Bibliographical note

Funding Information:
Funding This material is based upon work supported by the National Science Foundation CAREER Award grant number 2045853. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.

Funding Information:
We gratefully acknowledge NIH Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation (CPRI, P20GM130456) and NIH NIDCR funding (R03DE029547) for completion of these experiments. Funding This material is based upon work supported by the National Science Foundation CAREER Award grant number 2045853. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.

Publisher Copyright:
© 2023, The Society for Experimental Mechanics, Inc.

Keywords

Adhesion
Biofilms
Dental implants
Laser spallation

ASJC Scopus subject areas

Engineering (all)
Computational Mechanics
Mechanical Engineering

Access to Document

10.1007/978-3-031-17457-5_11

Cite this

Hessin, M. N., Boyd, J. D., & Grady, M. E. (2023). Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface. In A. Amirkhizi, J. Furmanski, C. Franck, K. Kasza, A. Forster, & J. Estrada (Eds.), Challenges in Mechanics of Time-Dependent Materials and Mechanics of Biological Systems and Materials, Volume 2 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics (pp. 71-75). (Conference Proceedings of the Society for Experimental Mechanics Series). https://doi.org/10.1007/978-3-031-17457-5_11

Hessin, M. N. ; Boyd, J. D. ; Grady, M. E. / Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface. Challenges in Mechanics of Time-Dependent Materials and Mechanics of Biological Systems and Materials, Volume 2 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics. editor / Alireza Amirkhizi ; Jevan Furmanski ; Christian Franck ; Karen Kasza ; Aaron Forster ; Jon Estrada. 2023. pp. 71-75 (Conference Proceedings of the Society for Experimental Mechanics Series).

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title = "Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface",

abstract = "Adhesion of bacteria to oral implant surfaces can lead to oral infections, and the prevention of strong biofilm adherence to implant surfaces can assist in the prevention of these infections like peri-implantitis. In prior studies, single species biofilm adhesion has been quantitatively measured via the laser spallation technique. However, colonizing oral biofilms rarely consists of a single bacteria species. Multiple early colonizer species, including several strains of Streptococci, dominate initial oral biofilm formation. This study aims to characterize the adhesion of a multi-species oral biofilm consisting of S. oralis, S. sanguinis, and S. gordonii on titanium, a common implant material, using the laser spallation technique. Previous work has established these specific Streptococci strains as a multi-species periodontal biofilm model. This study is the first to provide a quantitative adhesion measurement of this multi-species model onto a dental implant surface. First, adhesion strength of the multi-species model is compared to adhesion strength of the single-species streptococci constituents. Fluorescent staining and imaging by fluorescent microscopy are used to identify individual bacteria species within the biofilm. The multi-species biofilm presented in this study provides a more representative model of in vivo early biofilms and provides a more accurate metric for understanding biocompatibility on implant surfaces.",

keywords = "Adhesion, Biofilms, Dental implants, Laser spallation",

author = "Hessin, {M. N.} and Boyd, {J. D.} and Grady, {M. E.}",

note = "Funding Information: Funding This material is based upon work supported by the National Science Foundation CAREER Award grant number 2045853. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. Funding Information: We gratefully acknowledge NIH Center of Biomedical Research Excellence (COBRE) in Pharmaceutical Research and Innovation (CPRI, P20GM130456) and NIH NIDCR funding (R03DE029547) for completion of these experiments. Funding This material is based upon work supported by the National Science Foundation CAREER Award grant number 2045853. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. Publisher Copyright: {\textcopyright} 2023, The Society for Experimental Mechanics, Inc.; null ; Conference date: 13-06-2022 Through 16-06-2022",

year = "2023",

doi = "10.1007/978-3-031-17457-5_11",

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pages = "71--75",

editor = "Alireza Amirkhizi and Jevan Furmanski and Christian Franck and Karen Kasza and Aaron Forster and Jon Estrada",

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Hessin, MN, Boyd, JD & Grady, ME 2023, Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface. in A Amirkhizi, J Furmanski, C Franck, K Kasza, A Forster & J Estrada (eds), Challenges in Mechanics of Time-Dependent Materials and Mechanics of Biological Systems and Materials, Volume 2 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics. Conference Proceedings of the Society for Experimental Mechanics Series, pp. 71-75, SEM Annual Conference and Exposition on Experimental and Applied Mechanics, 2022, Pittsburgh, United States, 6/13/22. https://doi.org/10.1007/978-3-031-17457-5_11

Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface. / Hessin, M. N.; Boyd, J. D.; Grady, M. E.

Challenges in Mechanics of Time-Dependent Materials and Mechanics of Biological Systems and Materials, Volume 2 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics. ed. / Alireza Amirkhizi; Jevan Furmanski; Christian Franck; Karen Kasza; Aaron Forster; Jon Estrada. 2023. p. 71-75 (Conference Proceedings of the Society for Experimental Mechanics Series).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - Adhesion of bacteria to oral implant surfaces can lead to oral infections, and the prevention of strong biofilm adherence to implant surfaces can assist in the prevention of these infections like peri-implantitis. In prior studies, single species biofilm adhesion has been quantitatively measured via the laser spallation technique. However, colonizing oral biofilms rarely consists of a single bacteria species. Multiple early colonizer species, including several strains of Streptococci, dominate initial oral biofilm formation. This study aims to characterize the adhesion of a multi-species oral biofilm consisting of S. oralis, S. sanguinis, and S. gordonii on titanium, a common implant material, using the laser spallation technique. Previous work has established these specific Streptococci strains as a multi-species periodontal biofilm model. This study is the first to provide a quantitative adhesion measurement of this multi-species model onto a dental implant surface. First, adhesion strength of the multi-species model is compared to adhesion strength of the single-species streptococci constituents. Fluorescent staining and imaging by fluorescent microscopy are used to identify individual bacteria species within the biofilm. The multi-species biofilm presented in this study provides a more representative model of in vivo early biofilms and provides a more accurate metric for understanding biocompatibility on implant surfaces.

KW - Adhesion

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Hessin MN, Boyd JD, Grady ME. Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface. In Amirkhizi A, Furmanski J, Franck C, Kasza K, Forster A, Estrada J, editors, Challenges in Mechanics of Time-Dependent Materials and Mechanics of Biological Systems and Materials, Volume 2 - Proceedings of the 2022 Annual Conference on Experimental and Applied Mechanics. 2023. p. 71-75. (Conference Proceedings of the Society for Experimental Mechanics Series). doi: 10.1007/978-3-031-17457-5_11

Measuring Adhesion Strength of an Improved Dental Biofilm Model on a Titanium Surface

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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