Measuring FRET efficiency with phase lifetime

Daniel Sumetsky; James Y. Jiang; Marina Ayad; Timothy Mahon; Audrey Menaesse; Marina M. Cararo-Lopes; Mihir V. Patel; Bonnie L. Firestein; Nada N. Boustany

Measuring FRET efficiency with phase lifetime

Daniel Sumetsky
, James Y. Jiang
, Marina Ayad
, Timothy Mahon
, Audrey Menaesse
, Marina M. Cararo-Lopes
, Mihir V. Patel
, Bonnie L. Firestein
, Nada N. Boustany

Pharmacology and Nutritional Sciences

Research output: Contribution to journal › Conference article › peer-review

Abstract

A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.

Original language	English
Article number	MTu4A.4
Journal	Optics InfoBase Conference Papers
State	Published - 2022
Event	Microscopy Histopathology and Analytics, Microscopy 2022 - Fort Lauderdale, United States Duration: Apr 24 2022 → Apr 27 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s).

Funding

Acknowledgements: This study was supported by a NSF GOALI grant under award CMMI-1825433 to N.N.B and B.L.F. M.C.C. was supported by a Predoctoral Fellowship from CAPES, and M.P. was supported by NJCBIR Predoctoral Fellowship #CBIR15FEL009.

Funders	Funder number
National Science Foundation	CMMI-1825433
New Jersey Commission on Brain Injury Research	15FEL009
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Mechanics of Materials

Cite this

@article{197fa86399a740258aeefadd21d2071c,

title = "Measuring FRET efficiency with phase lifetime",

abstract = "A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.",

author = "Daniel Sumetsky and Jiang, \{James Y.\} and Marina Ayad and Timothy Mahon and Audrey Menaesse and Cararo-Lopes, \{Marina M.\} and Patel, \{Mihir V.\} and Firestein, \{Bonnie L.\} and Boustany, \{Nada N.\}",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s).; Microscopy Histopathology and Analytics, Microscopy 2022 ; Conference date: 24-04-2022 Through 27-04-2022",

year = "2022",

language = "English",

}

TY - JOUR

T1 - Measuring FRET efficiency with phase lifetime

AU - Sumetsky, Daniel

AU - Jiang, James Y.

AU - Ayad, Marina

AU - Mahon, Timothy

AU - Menaesse, Audrey

AU - Cararo-Lopes, Marina M.

AU - Patel, Mihir V.

AU - Firestein, Bonnie L.

AU - Boustany, Nada N.

PY - 2022

Y1 - 2022

N2 - A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.

AB - A linear relationship between apparent EFRET app, based on phase lifetime, and EFRET true, based on sensitized emission, holds for modulation frequencies such that 1.1 and simplifies data acquisition and interpretation in FLIM-FRET.

UR - https://www.scopus.com/pages/publications/85136440344

UR - https://www.scopus.com/pages/publications/85136440344#tab=citedBy

M3 - Conference article

AN - SCOPUS:85136440344

JO - Optics InfoBase Conference Papers

JF - Optics InfoBase Conference Papers

M1 - MTu4A.4

T2 - Microscopy Histopathology and Analytics, Microscopy 2022

Y2 - 24 April 2022 through 27 April 2022

ER -

Measuring FRET efficiency with phase lifetime

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

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