Measuring the impact of microenvironmental conditions on mitochondrial dehydrogenase activity in cultured cells

Ramon C. Sun, Albert Koong, Amato Giaccia, Nicholas C. Denko

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Mitochondria are powerhouses of a cell, producing much of the cellular ATP. However, mitochondrial enzymes also participate in many cellular biosynthetic processes. They are responsible for helping to maintain NAD(P)/H and redox balance, supplying metabolic intermediates for cell growth, and regulating several types of programed cell death. Several mitochondrial enzymes have even been shown to participate in the oncogenic process such as isocitrate dehydrogenase, succinate dehydrogenase, and fumarate hydratase. Recent advances have identified significant metabolic changes in the mitochondria that are regulated by malignant transformation and environmental stimuli. Understanding the biological activity and regulation of mitochondrial enzymes can provide insight into how they participate in the process of oncogenic transformation and work to sustain malignant growth. This chapter describes a technique to measure mitochondrial dehydrogenase activities that is faster and more cost effective which can also be scaled up for high throughput.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
Pages113-120
Number of pages8
DOIs
StatePublished - 2016

Publication series

NameAdvances in Experimental Medicine and Biology
Volume899
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Bibliographical note

Publisher Copyright:
© Springer International Publishing Switzerland 2016.

Keywords

  • Colorimetric detection
  • Dehydrogenase
  • Enzymatic activity
  • Hypoglycemia
  • Hypoxia

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology

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