Mechanical properties and dual drug delivery application of poly(lactic-co-glycolic acid) scaffolds fabricated with a poly(β-amino ester) porogen

Amanda Clark, Todd A. Milbrandt, J. Zach Hilt, David A. Puleo

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Polymeric scaffolds that are biocompatible and biodegradable are widely used for tissue engineering applications. Scaffolds can be further enhanced by enabling the release of one or more drugs to stimulate regeneration or for the treatment of a specific disease or condition. In this study, poly(lactic-co-glycolic acid) (PLGA) microspheres were mixed with poly(β-amino ester) (PBAE) particles to create novel hybrid scaffolds capable of dual release of drug and growth factor. Fast-degrading PBAE particles loaded with the drug ketoprofen acted as porogens that provided a rapid 12 h release. The PLGA microspheres were loaded with a growth factor, bone morphogenetic protein 2, and fused together around the porogens to create a slow-degrading matrix that provided sustained release lasting 70 days. Drug release was further tailored by varying the amount of porogen added to the scaffold. Bioactivity measurements demonstrated that the scaffold fabrication technique did not damage the drug or protein. The compressive modulus was affected by the amount of porogen added, extending from 50 to 111 MPa for loadings from 60 to 40% PBAE, and after 5 days of degradation, it decreased to 0.6 to 1.1 kPa when the porogen was gone. PLGA containing a quick-degrading porogen can be used to release two drugs while developing a porous microarchitecture for cell ingrowth with in a matrix capable of maintaining a compressive modulus applicable for soft tissue implants.

Original languageEnglish
Pages (from-to)2125-2132
Number of pages8
JournalActa Biomaterialia
Volume10
Issue number5
DOIs
StatePublished - May 2014

Bibliographical note

Funding Information:
This research was supported in part by Kosair Charities, Inc., the Pediatric Orthopaedic Society of North America, the NSF (EPS-0814194) and an NSF IGERT traineeship to AC (DGE-0653710).

Keywords

  • Drug delivery
  • Poly(lactic-co-glycolic acid)
  • Poly(β-amino ester)
  • Porogen
  • Scaffolds

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Mechanical properties and dual drug delivery application of poly(lactic-co-glycolic acid) scaffolds fabricated with a poly(β-amino ester) porogen'. Together they form a unique fingerprint.

Cite this