TY - JOUR
T1 - Mechanism of hsp70i gene bookmarking
AU - Xing, Hongyan
AU - Wilkerson, Donald C.
AU - Mayhew, Christopher N.
AU - Lubert, Eric J.
AU - Skaggs, Hollie S.
AU - Goodson, Michael L.
AU - Hong, Yiling
AU - Park-Sarge, Ok Kyong
AU - Sarge, Kevin D.
PY - 2005/1/21
Y1 - 2005/1/21
N2 - In contrast to most genomic DNA in mitotic cells, the promoter regions of some genes, such as the stress-inducible hsp70i gene that codes for a heat shock protein, remain uncompacted, a phenomenon called bookmarking. Here we show that hsp70i bookmarking is mediated by a transcription factor called HSF2, which binds this promoter in mitotic cells, recruits protein phosphatase 2A, and interacts with the CAP-G subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. Blocking HSF2-mediated bookmarking by HSF2 RNA interference decreases hsp70i induction and survival of stressed cells in the G1 phase, which demonstrates the biological importance of gene bookmarking.
AB - In contrast to most genomic DNA in mitotic cells, the promoter regions of some genes, such as the stress-inducible hsp70i gene that codes for a heat shock protein, remain uncompacted, a phenomenon called bookmarking. Here we show that hsp70i bookmarking is mediated by a transcription factor called HSF2, which binds this promoter in mitotic cells, recruits protein phosphatase 2A, and interacts with the CAP-G subunit of the condensin enzyme to promote efficient dephosphorylation and inactivation of condensin complexes in the vicinity, thereby preventing compaction at this site. Blocking HSF2-mediated bookmarking by HSF2 RNA interference decreases hsp70i induction and survival of stressed cells in the G1 phase, which demonstrates the biological importance of gene bookmarking.
UR - https://www.scopus.com/pages/publications/12344307185
UR - https://www.scopus.com/pages/publications/12344307185#tab=citedBy
U2 - 10.1126/science.1106478
DO - 10.1126/science.1106478
M3 - Article
C2 - 15662014
AN - SCOPUS:12344307185
SN - 0036-8075
VL - 307
SP - 421
EP - 423
JO - Science
JF - Science
IS - 5708
ER -