Mechanistic implications of Escherichia coli galactokinase structure-based engineering

Dirk Hoffmeister; Jon S. Thorson

doi:10.1002/cbic.200400003

Mechanistic implications of Escherichia coli galactokinase structure-based engineering

Dirk Hoffmeister, Jon S. Thorson

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Engineering GalK: The Escherichia coli galactokinase GalK has been engineered in its active site. The amino acid exchanges Y223F and Y223W broadened the sugar-substrate spectrum to include, among others, 4-deoxy-D-galactose. This finding suggests that the role of Y223 is not primarily hydrogen bonding but rather a hydrophobic stacking to secure the hexopyranose ring in the active site.

Original language	English
Pages (from-to)	989-992
Number of pages	4
Journal	ChemBioChem
Volume	5
Issue number	7
DOIs	https://doi.org/10.1002/cbic.200400003
State	Published - Jul 5 2004

Keywords

Carbohydrates
Enzymes
Glycorandomization
Kinases
Phosphosugars

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200400003

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abstract = "Engineering GalK: The Escherichia coli galactokinase GalK has been engineered in its active site. The amino acid exchanges Y223F and Y223W broadened the sugar-substrate spectrum to include, among others, 4-deoxy-D-galactose. This finding suggests that the role of Y223 is not primarily hydrogen bonding but rather a hydrophobic stacking to secure the hexopyranose ring in the active site.",

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AB - Engineering GalK: The Escherichia coli galactokinase GalK has been engineered in its active site. The amino acid exchanges Y223F and Y223W broadened the sugar-substrate spectrum to include, among others, 4-deoxy-D-galactose. This finding suggests that the role of Y223 is not primarily hydrogen bonding but rather a hydrophobic stacking to secure the hexopyranose ring in the active site.

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KW - Glycorandomization

KW - Kinases

KW - Phosphosugars

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Mechanistic implications of Escherichia coli galactokinase structure-based engineering

Abstract

Keywords

ASJC Scopus subject areas

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