Mechanistic implications of Escherichia coli galactokinase structure-based engineering

Dirk Hoffmeister, Jon S. Thorson

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Engineering GalK: The Escherichia coli galactokinase GalK has been engineered in its active site. The amino acid exchanges Y223F and Y223W broadened the sugar-substrate spectrum to include, among others, 4-deoxy-D-galactose. This finding suggests that the role of Y223 is not primarily hydrogen bonding but rather a hydrophobic stacking to secure the hexopyranose ring in the active site.

Original languageEnglish
Pages (from-to)989-992
Number of pages4
Issue number7
StatePublished - Jul 5 2004


  • Carbohydrates
  • Enzymes
  • Glycorandomization
  • Kinases
  • Phosphosugars

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry


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