Abstract
Existing biosensors employ two major components: analyte recognition and signal transduction. Although specificity is achieved through analyte recognition, sensitivity is usually enhanced through a chemical amplification stage that couples the two main units in a sensor. Although highly sensitive, the extra chemical amplification stage complicates the sensing protocol. In addition, it separates the two elements spatiotemporally, reducing the real-time response of the biosensor. In this review, we discuss the new mechanochemical biosensors that employ mechanochemical coupling strategies to overcome these issues. By monitoring changes in the mechanical properties of a single-molecule template upon analyte binding, single-molecule sensitivity is reached. As chemical amplification becomes unnecessary in this single-molecule mechanochemical sensing (SMMS) strategy, real-time sensing is achieved. One spanner short New mechanochemical biosensors that employ mechanochemical coupling strategies to overcome the issues of existing biosensors are reviewed. By monitoring changes in the mechanical properties of a single-molecule template upon analyte binding, single-molecule sensitivity can be reached.
Original language | English |
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Pages (from-to) | 1829-1837 |
Number of pages | 9 |
Journal | ChemPhysChem |
Volume | 16 |
Issue number | 9 |
DOIs | |
State | Published - Jun 1 2015 |
Bibliographical note
Publisher Copyright:© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Keywords
- biosensors
- DNA
- mechanochemical sensing
- optical tweezers
- single-molecule techniques
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
- Physical and Theoretical Chemistry