Mechanosensitive PPAP2B regulates endothelial responses to atherorelevant hemodynamic forces

Congqing Wu, Ru Ting Huang, Cheng Hsiang Kuo, Sandeep Kumar, Chan Woo Kim, Yen Chen Lin, Yen Ju Chen, Anna Birukova, Konstantin G. Birukov, Nickolai O. Dulin, Mete Civelek, Aldons J. Lusis, Xavier Loyer, Alain Tedgui, Guohao Dai, Hanjoong Jo, Yun Fang

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


Rationale: PhosPhatidic Acid Phosphatase type 2B (PPAP2B), an integral membrane protein known as lipid phosphate phosphatase (LPP3) that inactivates lysophosphatidic acid, was implicated in coronary artery disease (CAD) by genomewide association studies. However, it is unclear whether genome-wide association studies-identified coronary artery disease genes, including PPAP2B, participate in mechanotransduction mechanisms by which vascular endothelia respond to local atherorelevant hemodynamics that contribute to the regional nature of atherosclerosis. Objective: To establish the critical role of PPAP2B in endothelial responses to hemodynamics. Methods and Results: Reduced PPAP2B was detected in vivo in mouse and swine aortic arch (AA) endothelia exposed to chronic disturbed flow, and in mouse carotid artery endothelia subjected to surgically induced acute disturbed flow. In humans, PPAP2B was reduced in the downstream part of carotid plaques where low shear stress prevails. In culture, reduced PPAP2B was measured in human aortic endothelial cells under atherosusceptible waveform mimicking flow in human carotid sinus. Flow-sensitive microRNA-92a and transcription factor KLF2 were identified as upstream inhibitor and activator of endothelial PPAP2B, respectively. PPAP2B suppression abrogated atheroprotection of unidirectional flow; inhibition of lysophosphatidic acid receptor 1 restored the flow-dependent, anti-inflammatory phenotype in PPAP2B-deficient cells. PPAP2B inhibition resulted in myosin light-chain phosphorylation and intercellular gaps, which were abolished by lysophosphatidic acid receptor 1/2 inhibition. Expression quantitative trait locus mapping demonstrated PPAP2B coronary artery disease risk allele is not linked to PPAP2B expression in various human tissues but significantly associated with reduced PPAP2B in human aortic endothelial cells. Conclusions: Atherorelevant flows dynamically modulate endothelial PPAP2B expression through miR-92a and KLF2. Mechanosensitive PPAP2B plays a critical role in promoting anti-inflammatory phenotype and maintaining vascular integrity of endothelial monolayer under atheroprotective flow.

Original languageEnglish
Pages (from-to)e41-e53
JournalCirculation Research
Issue number4
StatePublished - Jul 1 2015

Bibliographical note

Publisher Copyright:
© 2015 American Heart Association, Inc.


  • Atherosclerosis
  • Endothelial cells
  • Genome wide association study
  • Hemodynamics
  • MicroRNAs
  • Permeability

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine


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