Membrane-based scintillation proximity assays I. Detection and quantification of 14CO2

Colin D. Mattingly, Robert K. Mansfield, Dibakar Bhattacharyya, Michael Jay

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


14CO2 is a radioactive byproduct of numerous clinical exams and enzymatic assays. Traditional methods for capturing and quantifying 14CO2 produce a large quantity of radioactive liquid organic waste, which is both difficult and expensive to handle. A polymeric membrane which can simultaneously capture and quantify 14CO2 has been developed to circumvent both problems. The membrane contains a quaternary amine trapping agent (HyamineTM) and an organic fluor for β- detection. A 12% (w/v) polysulfone/sulfonated polysulfone blend membrane containing 0.5 mmol of HyamineTM captured 4.0 × 10-5 mmol/cm2 of 14CO2 with a counting efficiency of 70%. The membrane retained all of the 14CO2 for at least a three-day period.

Original languageEnglish
Pages (from-to)275-280
Number of pages6
JournalJournal of Membrane Science
Issue number3
StatePublished - Jan 31 1995

Bibliographical note

Copyright 2014 Elsevier B.V., All rights reserved.


  • Affinity membranes
  • Microporous and porous membranes

ASJC Scopus subject areas

  • Biochemistry
  • Materials Science (all)
  • Physical and Theoretical Chemistry
  • Filtration and Separation


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