Membrane protein alterations in rodent erythrocytes and synaptosomes due to aging and hyperoxia

Kenneth Hensley, Beverly J. Howard, John M. Carney, D. Allan Butterfield

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


We have applied the technique of electron paramagnetic resonance (EPR) protein-specific spin labeling to the study of membrane protein alterations occurring during age and exposure to isobaric hyperoxia. Cortical synaptosomes and erythrocyte membranes (ghosts) were isolated from young rodents (Fisher 344 rats or mongolian gerbils, 3-4 months of age) and aged rodents (age 22-27 months for rats, greater than 15 months for gerbils). Membrane proteins were spin labeled with the thiol-specific spin label MAL-6 (2,2,6,6,-tetramethyl-4-maleimido-piperdin-1-oxyl). The relevant EPR spectral parameter of MAL-6 labeled membranes, the W/S ratio, decreased significantly with age of animal in both synaptosomes and ghosts (P < 0.001). As a paradigm for accelerated oxidative stress, young and aged gerbils were exposed to an atmosphere of 90-100% O2 for 0-48 h. In both young and aged gerbils, the W/S ratio decreased significantly with hyperoxic stress (P < 0.003). The W/S ratio of synaptosomes isolated from aged gerbils decreased continually from 0-48 h hyperoxia, whereas the W/S ratio of synaptosomes from young animals demonstrated a pronounced rebound effect from 24-48 h. The results are discussed with reference to membrane protein oxidation in aging.

Original languageEnglish
Pages (from-to)203-206
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Issue number2-3
StatePublished - Apr 24 1995

Bibliographical note

Funding Information:
This work was supported in part by grants from the National Institutes of Health (AG-10836) and the National Science Foundation (CTS-9307518).


  • Aging
  • Free radicals
  • Hyperoxia
  • Membrane protein
  • Protein oxidation
  • Spin labeling

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology


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