Metabolic and transcriptional regulation of reproductive diapause in Arma chinensis

Maosen Zhang; Weiwei He; Yuyan Li; Junjie Chen; Nicholas M. Teets; Lisheng Zhang

doi:10.1016/j.isci.2025.111761

Metabolic and transcriptional regulation of reproductive diapause in Arma chinensis

Maosen Zhang
, Weiwei He
, Yuyan Li
, Junjie Chen
, Nicholas M. Teets
, Lisheng Zhang

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Diapause enables insects to survive unfavorable conditions through metabolic and developmental adjustments. We investigated metabolic regulation during reproductive diapause in the predatory stinkbug Arma chinensis using transcriptomic and metabolomic analyses. Our study revealed 9,254 differentially expressed genes and 493 significantly changed metabolites across diapause stages. Key metabolic pathways including glutathione metabolism, TCA cycle, glycolysis, and lipid metabolism underwent substantial reorganization. The pre-diapause phase showed increased energy consumption and lipid accumulation, while the maintenance phase exhibited restructuring of amino acid and glucose metabolism. We identified stage-specific metabolic signatures and potential regulatory mechanisms, including the roles of glutathione metabolism in redox regulation and insulin signaling in diapause control. This comprehensive characterization of metabolic reprogramming during A. chinensis diapause provides insights for improving biocontrol agent production and storage strategies.

Original language	English
Article number	111761
Journal	iScience
Volume	28
Issue number	3
DOIs	https://doi.org/10.1016/j.isci.2025.111761
State	Published - Mar 21 2025

Bibliographical note

Publisher Copyright:
© 2025

Funding

This research was funded by National Key R&D Program of China (2021YFD1400703, 2023YFE0123000), the Major Project of China National Tobacco Corporation (110202201022 (LS-06)) and Guizhou Provincial Tobacco Corporation Project (2024XM07).

Funders	Funder number
Major Project of China National Tobacco Corporation	110202201022, LS-06
National Key Basic Research and Development Program of China	2023YFE0123000, 2021YFD1400703
National Key Basic Research and Development Program of China
Guizhou Provincial Tobacco Corporation Project	2024XM07

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

Keywords

Entomology
Genetics
Omics

ASJC Scopus subject areas

General

Access to Document

10.1016/j.isci.2025.111761

Cite this

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title = "Metabolic and transcriptional regulation of reproductive diapause in Arma chinensis",

abstract = "Diapause enables insects to survive unfavorable conditions through metabolic and developmental adjustments. We investigated metabolic regulation during reproductive diapause in the predatory stinkbug Arma chinensis using transcriptomic and metabolomic analyses. Our study revealed 9,254 differentially expressed genes and 493 significantly changed metabolites across diapause stages. Key metabolic pathways including glutathione metabolism, TCA cycle, glycolysis, and lipid metabolism underwent substantial reorganization. The pre-diapause phase showed increased energy consumption and lipid accumulation, while the maintenance phase exhibited restructuring of amino acid and glucose metabolism. We identified stage-specific metabolic signatures and potential regulatory mechanisms, including the roles of glutathione metabolism in redox regulation and insulin signaling in diapause control. This comprehensive characterization of metabolic reprogramming during A. chinensis diapause provides insights for improving biocontrol agent production and storage strategies.",

keywords = "Entomology, Genetics, Omics",

author = "Maosen Zhang and Weiwei He and Yuyan Li and Junjie Chen and Teets, \{Nicholas M.\} and Lisheng Zhang",

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doi = "10.1016/j.isci.2025.111761",

language = "English",

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AU - Zhang, Maosen

AU - He, Weiwei

AU - Li, Yuyan

AU - Chen, Junjie

AU - Teets, Nicholas M.

AU - Zhang, Lisheng

PY - 2025/3/21

Y1 - 2025/3/21

N2 - Diapause enables insects to survive unfavorable conditions through metabolic and developmental adjustments. We investigated metabolic regulation during reproductive diapause in the predatory stinkbug Arma chinensis using transcriptomic and metabolomic analyses. Our study revealed 9,254 differentially expressed genes and 493 significantly changed metabolites across diapause stages. Key metabolic pathways including glutathione metabolism, TCA cycle, glycolysis, and lipid metabolism underwent substantial reorganization. The pre-diapause phase showed increased energy consumption and lipid accumulation, while the maintenance phase exhibited restructuring of amino acid and glucose metabolism. We identified stage-specific metabolic signatures and potential regulatory mechanisms, including the roles of glutathione metabolism in redox regulation and insulin signaling in diapause control. This comprehensive characterization of metabolic reprogramming during A. chinensis diapause provides insights for improving biocontrol agent production and storage strategies.

AB - Diapause enables insects to survive unfavorable conditions through metabolic and developmental adjustments. We investigated metabolic regulation during reproductive diapause in the predatory stinkbug Arma chinensis using transcriptomic and metabolomic analyses. Our study revealed 9,254 differentially expressed genes and 493 significantly changed metabolites across diapause stages. Key metabolic pathways including glutathione metabolism, TCA cycle, glycolysis, and lipid metabolism underwent substantial reorganization. The pre-diapause phase showed increased energy consumption and lipid accumulation, while the maintenance phase exhibited restructuring of amino acid and glucose metabolism. We identified stage-specific metabolic signatures and potential regulatory mechanisms, including the roles of glutathione metabolism in redox regulation and insulin signaling in diapause control. This comprehensive characterization of metabolic reprogramming during A. chinensis diapause provides insights for improving biocontrol agent production and storage strategies.

KW - Entomology

KW - Genetics

KW - Omics

UR - https://www.scopus.com/pages/publications/85219532836

UR - https://www.scopus.com/pages/publications/85219532836#tab=citedBy

U2 - 10.1016/j.isci.2025.111761

DO - 10.1016/j.isci.2025.111761

M3 - Article

AN - SCOPUS:85219532836

SN - 2589-0042

VL - 28

JO - iScience

JF - iScience

IS - 3

M1 - 111761

ER -

Metabolic and transcriptional regulation of reproductive diapause in Arma chinensis

Abstract

Bibliographical note

Funding

UN SDGs

Keywords

ASJC Scopus subject areas

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