Stable isotope-resolved metabolomics (SIRM) methods are used increasingly by cancer researchers to probe metabolic pathways and identify vulnerabilities in cancer cells. Analytical and computational advances are being made constantly, but tissue culture and sample extraction procedures are often variable and not elaborated in the literature. This chapter discusses basic aspects of tissue culture practices as they relate to the use of stable isotope tracers and provides a detailed metabolic labeling and metabolite extraction procedure designed to maximize the amount of information that can be obtained from a single tracer experiment.
|Title of host publication||Methods in Molecular Biology|
|Number of pages||27|
|State||Published - 2019|
|Name||Methods in Molecular Biology|
Bibliographical notePublisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
- Metabolite extraction
- Stable isotopes
- Tissue culture
ASJC Scopus subject areas
- Molecular Biology