Metabolic labeling of cultured mammalian cells for stable isotope-resolved metabolomics: Practical aspects of tissue culture and sample extraction

Daniel R. Crooks; Teresa W.M. Fan; W. Marston Linehan

doi:10.1007/978-1-4939-9027-6_1

Metabolic labeling of cultured mammalian cells for stable isotope-resolved metabolomics: Practical aspects of tissue culture and sample extraction

Daniel R. Crooks, Teresa W.M. Fan, W. Marston Linehan

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

13 Scopus citations

Abstract

Stable isotope-resolved metabolomics (SIRM) methods are used increasingly by cancer researchers to probe metabolic pathways and identify vulnerabilities in cancer cells. Analytical and computational advances are being made constantly, but tissue culture and sample extraction procedures are often variable and not elaborated in the literature. This chapter discusses basic aspects of tissue culture practices as they relate to the use of stable isotope tracers and provides a detailed metabolic labeling and metabolite extraction procedure designed to maximize the amount of information that can be obtained from a single tracer experiment.

Original language	English
Title of host publication	Methods in Molecular Biology
Pages	1-27
Number of pages	27
DOIs	https://doi.org/10.1007/978-1-4939-9027-6_1
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1928
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

Glucose
Glutamine
Metabolite extraction
Metabolomics
Stable isotopes
Tissue culture

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/978-1-4939-9027-6_1

Cite this

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Metabolic labeling of cultured mammalian cells for stable isotope-resolved metabolomics: Practical aspects of tissue culture and sample extraction. / Crooks, Daniel R.; Fan, Teresa W.M.; Linehan, W. Marston.
Methods in Molecular Biology. 2019. p. 1-27 (Methods in Molecular Biology; Vol. 1928).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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KW - Stable isotopes

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Metabolic labeling of cultured mammalian cells for stable isotope-resolved metabolomics: Practical aspects of tissue culture and sample extraction

Abstract

Publication series

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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