Metabolic labeling of cultured mammalian cells for stable isotope-resolved metabolomics: Practical aspects of tissue culture and sample extraction

Daniel R. Crooks, Teresa W.M. Fan, W. Marston Linehan

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

6 Scopus citations

Abstract

Stable isotope-resolved metabolomics (SIRM) methods are used increasingly by cancer researchers to probe metabolic pathways and identify vulnerabilities in cancer cells. Analytical and computational advances are being made constantly, but tissue culture and sample extraction procedures are often variable and not elaborated in the literature. This chapter discusses basic aspects of tissue culture practices as they relate to the use of stable isotope tracers and provides a detailed metabolic labeling and metabolite extraction procedure designed to maximize the amount of information that can be obtained from a single tracer experiment.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages1-27
Number of pages27
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1928
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Keywords

  • Glucose
  • Glutamine
  • Metabolite extraction
  • Metabolomics
  • Stable isotopes
  • Tissue culture

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Metabolic labeling of cultured mammalian cells for stable isotope-resolved metabolomics: Practical aspects of tissue culture and sample extraction'. Together they form a unique fingerprint.

Cite this