Metabolism of 1, 2-Dimethylhydrazine by Cultured Rat Colon Epithelial Cells

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21 Scopus citations


The colon carcinogen 1,2-dimethylhydrazine (DMH) was cultured with rat colon epithelial cells to determine if these cells have the ability to metabolize DMH. Colon epithelial cells isolated from conventional and germfree Sprague-Dawley rats were incubated in CMRL 1066 supplemented medium containing 14 C-DMH. Cells from both groups of rats metabolized DMH to gaseous metabolites, to metabolites in the medium that were putatively identified as azoxymethane and methylazoxymethanol, and to products that bound to DNA. Cells from germfree rats metabolized DMH at an equal or greater rate than cells from conventional rats for the criteria examined. This report demonstrates that rat colon epithelial cells can metabolize DMH without previous metabolism by other tissues or colon bacteria.

Original languageEnglish
Pages (from-to)78-86
Number of pages9
JournalNutrition and Cancer
Issue number2
StatePublished - Jan 1 1983

Bibliographical note

Copyright 2016 Elsevier B.V., All rights reserved.

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research


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