Metabolites From Salivary Exosomes as Potential Biomarkers for Alveolar Bone and Tooth Resorption

W. J. Rody; L. M. Shaddox; K. Olli; A. Martinez-Porras; T. J. Garrett; J. G. Cagmat; L. S. Holliday

doi:10.1111/ocr.70024

Metabolites From Salivary Exosomes as Potential Biomarkers for Alveolar Bone and Tooth Resorption

W. J. Rody
, L. M. Shaddox
, K. Olli
, A. Martinez-Porras
, T. J. Garrett
, J. G. Cagmat
, L. S. Holliday

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Objective(s): In this pilot study, exosomes from saliva were isolated and tested for the presence of metabolomic biomarkers for physiological external root resorption and/or pathological alveolar bone resorption. Settings and Sample Population: Saliva samples of 20 individuals in the mixed dentition stage of dental development. Materials and Methods: Saliva was obtained from healthy control children with resorbing primary teeth or children with localised aggressive periodontitis (LAP) showing alveolar bone loss but little root resorption. Exosomes were isolated by differential centrifugation and analysed by electron microscopy, nanoparticle tracking analysis (NTA) and enzyme-linked immunosorbent assay (ELISA). Subsequently, exosomes were subjected to mass spectrometry to identify and quantitate metabolites. Differences between groups were statistically determined. Results: Over 2000 metabolites were detected in salivary exosomes. Metabolites that differed significantly between exosomes from the saliva of LAP patients and controls included L-pipecolic acid, acetylcholine, creatinine, N-acetylneuraminate and numerous unidentified molecules. Conclusion: This pilot study provided a proof-in-principle for using metabolites from salivary exosomes as biomarkers.

Original language	English
Pages (from-to)	S186-S193
Journal	Orthodontics and Craniofacial Research
Volume	28
Issue number	S1
DOIs	https://doi.org/10.1111/ocr.70024
State	Published - Dec 2025

Bibliographical note

Publisher Copyright:
© 2025 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Funding

Funding: Research reported in this publication was supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R03DE027504. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Research reported in this publication was supported by the National Institute of Dental and Craniofacial Research of the National Institutes of Health under Award Number R03DE027504. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding:

Funders	Funder number
National Institute of Dental and Craniofacial Research
National Institutes of Health (NIH)	R03DE027504

Keywords

exosomes
extracellular vesicles
localised aggressive periodontitis
metabolomics
root resorption
saliva

ASJC Scopus subject areas

Orthodontics
Surgery
Oral Surgery
Otorhinolaryngology

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10.1111/ocr.70024

Cite this

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title = "Metabolites From Salivary Exosomes as Potential Biomarkers for Alveolar Bone and Tooth Resorption",

abstract = "Objective(s): In this pilot study, exosomes from saliva were isolated and tested for the presence of metabolomic biomarkers for physiological external root resorption and/or pathological alveolar bone resorption. Settings and Sample Population: Saliva samples of 20 individuals in the mixed dentition stage of dental development. Materials and Methods: Saliva was obtained from healthy control children with resorbing primary teeth or children with localised aggressive periodontitis (LAP) showing alveolar bone loss but little root resorption. Exosomes were isolated by differential centrifugation and analysed by electron microscopy, nanoparticle tracking analysis (NTA) and enzyme-linked immunosorbent assay (ELISA). Subsequently, exosomes were subjected to mass spectrometry to identify and quantitate metabolites. Differences between groups were statistically determined. Results: Over 2000 metabolites were detected in salivary exosomes. Metabolites that differed significantly between exosomes from the saliva of LAP patients and controls included L-pipecolic acid, acetylcholine, creatinine, N-acetylneuraminate and numerous unidentified molecules. Conclusion: This pilot study provided a proof-in-principle for using metabolites from salivary exosomes as biomarkers.",

keywords = "exosomes, extracellular vesicles, localised aggressive periodontitis, metabolomics, root resorption, saliva",

author = "Rody, \{W. J.\} and Shaddox, \{L. M.\} and K. Olli and A. Martinez-Porras and Garrett, \{T. J.\} and Cagmat, \{J. G.\} and Holliday, \{L. S.\}",

note = "Publisher Copyright: {\textcopyright} 2025 John Wiley \& Sons A/S. Published by John Wiley \& Sons Ltd.",

year = "2025",

month = dec,

doi = "10.1111/ocr.70024",

language = "English",

volume = "28",

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T1 - Metabolites From Salivary Exosomes as Potential Biomarkers for Alveolar Bone and Tooth Resorption

AU - Rody, W. J.

AU - Shaddox, L. M.

AU - Olli, K.

AU - Martinez-Porras, A.

AU - Garrett, T. J.

AU - Cagmat, J. G.

AU - Holliday, L. S.

PY - 2025/12

Y1 - 2025/12

N2 - Objective(s): In this pilot study, exosomes from saliva were isolated and tested for the presence of metabolomic biomarkers for physiological external root resorption and/or pathological alveolar bone resorption. Settings and Sample Population: Saliva samples of 20 individuals in the mixed dentition stage of dental development. Materials and Methods: Saliva was obtained from healthy control children with resorbing primary teeth or children with localised aggressive periodontitis (LAP) showing alveolar bone loss but little root resorption. Exosomes were isolated by differential centrifugation and analysed by electron microscopy, nanoparticle tracking analysis (NTA) and enzyme-linked immunosorbent assay (ELISA). Subsequently, exosomes were subjected to mass spectrometry to identify and quantitate metabolites. Differences between groups were statistically determined. Results: Over 2000 metabolites were detected in salivary exosomes. Metabolites that differed significantly between exosomes from the saliva of LAP patients and controls included L-pipecolic acid, acetylcholine, creatinine, N-acetylneuraminate and numerous unidentified molecules. Conclusion: This pilot study provided a proof-in-principle for using metabolites from salivary exosomes as biomarkers.

AB - Objective(s): In this pilot study, exosomes from saliva were isolated and tested for the presence of metabolomic biomarkers for physiological external root resorption and/or pathological alveolar bone resorption. Settings and Sample Population: Saliva samples of 20 individuals in the mixed dentition stage of dental development. Materials and Methods: Saliva was obtained from healthy control children with resorbing primary teeth or children with localised aggressive periodontitis (LAP) showing alveolar bone loss but little root resorption. Exosomes were isolated by differential centrifugation and analysed by electron microscopy, nanoparticle tracking analysis (NTA) and enzyme-linked immunosorbent assay (ELISA). Subsequently, exosomes were subjected to mass spectrometry to identify and quantitate metabolites. Differences between groups were statistically determined. Results: Over 2000 metabolites were detected in salivary exosomes. Metabolites that differed significantly between exosomes from the saliva of LAP patients and controls included L-pipecolic acid, acetylcholine, creatinine, N-acetylneuraminate and numerous unidentified molecules. Conclusion: This pilot study provided a proof-in-principle for using metabolites from salivary exosomes as biomarkers.

KW - exosomes

KW - extracellular vesicles

KW - localised aggressive periodontitis

KW - metabolomics

KW - root resorption

KW - saliva

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JO - Orthodontics and Craniofacial Research

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ER -

Metabolites From Salivary Exosomes as Potential Biomarkers for Alveolar Bone and Tooth Resorption

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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