Method for handy rapid isolation of total RNA from cardiae tissue

With urea, a kind of protein denaturant and an inhibitor of ribonuclease, and LiCl which can seectively precipitates RNA we extracted total RNA from cardiac tissue. The quantity and quantity of extracted RNA are both satisfactory. No requirement for super-centrifugation and expensive agent such as Guanidinium thiocyanate and quanidine HCl is its advantage. Therefore the reported method is suitable for extraction of RNA in common laboratories.